In situ generation of cold atmospheric plasma-activated mist and its biocidal activity against surrogate viruses for COVID-19.
In situ generation of cold atmospheric plasma-activated mist and its biocidal activity against surrogate viruses for COVID-19.
复制标题
原位产生冷大气等离子体激活雾及其对 COVID-19 替代病毒的杀菌活性。
DOI：
10.1093/jambio/lxad181
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发表时间：
2023
影响因子：
4
通讯作者：
Upadrasta A
中科院分区：
文献类型：
--
作者：
Upadrasta A
AimsTo提供一种替代紫外线和汽化过氧化氢，以增强表面的去污作为应对COVID-19 pandemic.Methods and results的一部分，我们开发了一种间接方法，用于原位输送冷血浆，并使用噬菌体phi 6，MS 2和phiX 174（SARS-CoV-2的替代物）评估了血浆激活雾（PAM）的抗病毒活性。当PAM源位于5和10 cm处时，暴露于环境空气大气压时，PAM在5 min内导致phi 6滴度降低1.71 log 10 PFU ml − 1，10 min后降低7.4 log 10 PFU ml− 1。使用MS 2和phiX 174，30分钟后分别实现了3.1和1.26 log 10 PFU ml− 1的减少。杀灭率随着暴露时间的延长而增加，但随着PAM源的远离而降低。PAM中产生了痕量的活性物质，过氧化氢和亚硝酸盐，抗病毒活性可能归因于这些物质及其次级活性物质。
AimsTo provide an alternative to ultra violet light and vapourized hydrogen peroxide to enhance decontamination of surfaces as part of the response to the COVID-19 pandemic.Methods and resultsWe developed an indirect method forin situdelivery of cold plasma and evaluated the anti-viral activity of plasma-activated mist (PAM) using bacteriophages phi6, MS2, and phiX174, surrogates for SARS-CoV-2. Exposure to ambient air atmospheric pressure derived PAM caused a 1.71 log10PFU ml−1reduction in phi6 titer within 5 min and a 7.4 log10PFU ml−1reduction after 10 min when the the PAM source was at 5 and 10 cm. With MS2 and phiX174, a 3.1 and 1.26 log10PFU ml−1reduction was achieved, respectively, after 30 min. The rate of killing was increased with longer exposure times but decreased when the PAM source was further away. Trace amounts of reactive species, hydrogen peroxide and nitrite were produced in the PAM, and the anti-viral activity was probably attributable to these and their secondary reactive species.ConclusionsPAM exhibits virucidal activity against surrogate viruses for COVID-19, which is time and distance from the plasma source dependent.