Cell numbers and leaf development in Arabidopsis:: a functional analysis of the STRUWWELPETER gene
Cell numbers and leaf development in Arabidopsis:: a functional analysis of the STRUWWELPETER gene
复制标题
DOI：
10.1093/emboj/cdf614
复制
发表时间：
2002-11-15
期刊：
影响因子：
11.4
通讯作者：
Traas, J
中科院分区：
文献类型：
--
作者：
Autran, D;Jonak, C;Traas, J
拟南芥的Struwwell peter(SWP)突变体在所有气生器官中都显示出细胞数量减少。在某些情况下，这种缺陷可以通过增加最终单元大小来部分弥补。虽然突变不影响年轻原基中的细胞周期持续时间，但它确实影响了细胞增殖的窗口，因为在原基启动的非常早期阶段，细胞数量减少，细胞增殖早熟停止。此外，突变还扰乱了逐渐变得杂乱无章的顶端分生组织(SAM)。SWP编码一种与中介复合体亚单位相似的蛋白质，这是RNA聚合酶II在靶启动子对特定激活剂的反应中招募所必需的。为了进一步了解它的功能，我们在一个组成型启动子的控制下过度表达了该基因。这再次干扰了幼叶细胞周期停滞的时刻。我们的结果表明，SWP的水平除了在分生组织的模式形成中发挥作用外，在决定细胞增殖持续时间方面也发挥着重要作用。
The struwwelpeter (swp) mutant in Arabidopsis shows reduced cell numbers in all aerial organs. In certain cases, this defect is partially compensated by an increase in final cell size. Although the mutation does not affect cell cycle duration in the young primordia, it does influence the window of cell proliferation, as cell number is reduced during the very early stages of primordium initiation and a precocious arrest of cell proliferation occurs. In addition, the mutation also perturbs the shoot apical meristem (SAM), which becomes gradually disorganized. SWP encodes a protein with similarities to subunits of the Mediator complex, required for RNA polymerase II recruitment at target promoters in response to specific activators. To gain further insight into its function, we overexpressed the gene under the control of a constitutive promoter. This interfered again with the moment of cell cycle arrest in the young leaf. Our results suggest that the levels of SWP, besides their role in pattern formation at the meristem, play an important role in defining the duration of cell proliferation.