Evolution of a homopurine-homopyrimidine pentanucleotide repeat sequence upstream of the human inducible nitric oxide synthase gene
Evolution of a homopurine-homopyrimidine pentanucleotide repeat sequence upstream of the human inducible nitric oxide synthase gene
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DOI：
10.1016/s0378-1119(97)00538-6
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发表时间：
1997-12-19
期刊：
影响因子：
3.5
通讯作者：
Charles, IG
中科院分区：
文献类型：
--
作者：
Xu, WM;Liu, LZ;Charles, IG
我们在人诱导型一氧化氮合酶基因（iNOS，NOS 2）的5 '端启动子区域发现了一个高度多态的五核苷酸重复序列（CCTTT）n。使用一对来自人iNOS基因的特异性引物，我们还扩增了来自以下物种的DNA中的iNOS重复序列：黑猩猩，大猩猩，猩猩和猕猴。正如在人类身上发现的那样，黑猩猩和大猩猩在这个位点上都是多态的。相比之下，该基因座在猕猴和猩猩中是单态的。虽然大猩猩和人类的平均重复次数相似，但黑猩猩的重复次数要少得多。这些密切相关的物种之间的（CCTTT）n重复侧翼序列的比较表明存在长的高嘌呤-高嘧啶残基。类似的多嘌呤/多嘧啶延伸已被确定在许多其他脊椎动物基因的启动子区，在那里他们已经与转录调控，虽然（CCTTT）n重复阵列在人类iNOS基因的作用尚未得到证实。(C)1997年Elsevier Science B.V.
We have identified a highly polymorphic pentanucleotide repeat (CCTTT)n within the 5'-putative promoter region of the human inducible nitric oxide synthase gene (iNOS, NOS2). Using a pair of specific primers derived from the human iNOS gene, we have also amplified this iNOS repeat in DNA from the following species: chimpanzee, gorilla, orangutan and macaque. As is found in man, both chimpanzees and gorillas are polymorphic at this locus. In contrast, the locus is monomorphic in macaques and orangutans. While the average number of repeats is similar in gorilla and man, there are considerably fewer repeats in chimpanzees. A comparison of the sequences flanking the (CCTTT)n repeats among these closely related species demonstrates the presence of long stretches of homopurine-homopyrimidine residues. Similar polypurine/polypyrimidine stretches have been identified in the promoter regions of a number of other vertebrate genes where they have been associated with transcriptional regulation, although a role for the (CCTTT)n repeat array in the human iNOS gene has not yet been demonstrated. (C) 1997 Elsevier Science B.V.