In vitro toxicity of silica nanoparticles in human lung cancer cells
In vitro toxicity of silica nanoparticles in human lung cancer cells
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DOI：
10.1016/j.taap.2006.10.004
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发表时间：
2006-12-15
影响因子：
3.8
通讯作者：
Ma, Yinfa
中科院分区：
文献类型：
--
作者：
Lin, Weisheng;Huang, Yue-wern;Ma, Yinfa
以结晶二氧化硅（Min-U-Sil 5）为阳性对照，研究了15 nm和46 nm二氧化硅纳米颗粒对人支气管肺泡癌源性细胞的细胞毒性。暴露于15 nm或46 nm SiO2纳米颗粒48小时，剂量水平在10和100 μ g/ml之间，以剂量依赖性的方式降低细胞活力。两种SiO2纳米颗粒的细胞毒性均高于Min-U-Sil 5；然而，15 nm和46 nm二氧化硅纳米颗粒的细胞毒性无显著差异。15nm SiO2纳米颗粒用于测定细胞毒性和氧化应激反应的时间依赖性。细胞活力随纳米颗粒剂量（10-100 μ g/ml）和暴露时间（24小时、48小时和72小时）的变化而显著降低。定量评估氧化应激和细胞毒性指标，包括总活性氧（ROS）、谷胱甘肽、丙二醛和乳酸脱氢酶。暴露于二氧化硅纳米颗粒增加活性氧水平和谷胱甘肽水平降低。丙二醛和乳酸脱氢酶释放量的增加表明脂质过氧化和膜损伤。总之，暴露于二氧化硅纳米颗粒会导致培养的人支气管肺泡癌衍生细胞的剂量依赖性细胞毒性，这与氧化应激增加密切相关。(c) 2006爱思唯尔公司版权所有。
The cytotoxicity of 15-nm and 46-nm silica nanoparticles was investigated by using crystalline silica (Min-U-Sil 5) as a positive control in cultured human bronchoalveolar carcinoma-derived cells. Exposure to 15-nm or 46-nm SiO2 nanoparticles for 48 h at dosage levels between 10 and 100 mu g/ml decreased cell viability in a dose-dependent manner. Both SiO2 nanoparticles were more cytotoxic than Min-U-Sil 5; however, the cytotoxicities of 15-nm and 46-nm silica nanoparticles were not significantly different. The 15-nm SiO2 nanoparticles were used to determine time-dependent cytotoxicity and oxidative stress responses. Cell viability decreased significantly as a function of both nanoparticle dosage (10-100 mu g/ml) and exposure time (24 h, 48 h, and 72 h). Indicators of oxidative stress and cytotoxicity, including total reactive oxygen species (ROS), glutathione, malondialdehyde, and lactate dehydrogenase, were quantitatively assessed. Exposure to SiO2 nanoparticles increased ROS levels and reduced glutathione levels. The increased production of malondialdehyde and lactate dehydrogenase release from the cells indicated lipid peroxidation and membrane damage. In summary, exposure to SiO2 nanoparticles results in a dose-dependent cytotoxicity in cultural human bronchoalveolar carcinoma-derived cells that is closely correlated to increased oxidative stress. (c) 2006 Elsevier Inc. All rights reserved.