Phthalate esters reveal skin-sensitizing activity of phenethyl isothiocyanate in mice
Phthalate esters reveal skin-sensitizing activity of phenethyl isothiocyanate in mice
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DOI：
10.1016/j.fct.2010.03.049
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发表时间：
2010-06-01
影响因子：
4.3
通讯作者：
Imai, Yasuyuki
中科院分区：
文献类型：
--
作者：
Matsuda, Tomoko;Maruyama, Takashi;Imai, Yasuyuki
异硫氰酸苯乙酯（PEITC）是十字花科蔬菜的主要成分，由于其潜在的癌症化学预防活性而受到关注。虽然其蛋白结合活性是已知的，但PEITC尚未作为接触致敏剂进行研究。我们先前证明，邻苯二甲酸酯，包括邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二正丙酯（DPP），在异硫氰酸荧光素（FITC）诱导的接触性超敏反应小鼠模型中增强皮肤致敏性。在这项研究中，我们研究了DBP和DPP是否增强皮肤对FITC以外的半抗原的致敏性。在这项研究中，我们测试了PEITC作为接触致敏剂。BALB/c小鼠用次优剂量的半抗原（含或不含邻苯二甲酸酯）进行表皮致敏。用相应半抗原激发后的耳肿胀反应评价致敏性。DBP和DPP没有增强对两种辅助性T细胞1型（2，4-二硝基氟苯和恶唑酮）或三种辅助性T细胞2型（偏苯三酸酐、亚甲基二苯基4，4 '-二异氰酸酯和甲苯2，4-二异氰酸酯）半抗原的敏感性。相反，DBP和DPP增强了对两种FITC类似物（曙红5-异硫氰酸酯和罗丹明B异硫氰酸酯）以及PEITC的敏感性。在对除FITC以外的半抗原（包括食品成分PEITC）的接触性超敏反应中观察到DBP和DPP的佐剂作用。(C)2010爱思唯尔有限公司版权所有。
Phenethyl isothiocyanate (PEITC) is a constituent of edible cruciferous vegetables and has received attention due to its potential cancer chemopreventive activity. Although its protein-binding activity is known, PEITC has not been studied as a contact sensitizer. We previously demonstrated that phthalate esters, including dibutyl phthalate (DBP) and di-n-propyl phthalate (DPP), enhance skin sensitization in a fluorescein isothiocyanate (FITC)-induced contact hypersensitivity mouse model. In this study, we examined whether DBP and DPP enhance skin sensitization to haptens other than FITC. During this study, we tested PEITC as a contact sensitizer. BALB/c mice were epicutaneously sensitized with a suboptimal dose of a hapten with or without a phthalate ester. Sensitization was evaluated as the ear swelling response after a challenge with the respective hapten. DBP and DPP did not enhance the sensitization to two T-helper 1-type (2,4-dinitrofluorobenzene and oxazolone) or three T-helper 2-type (trimellitic anhydride, methylenediphenyl 4,4'-diisocyanate, and tolulene 2,4-diisocyanate) haptens. In contrast, DBP and DPP enhanced the sensitization to two FITC analogues (eosin 5-isothiocyanate and rhodamine B isothiocyanate) as well as to PEITC. Adjuvant effects of DBP and DPP were observed in contact hypersensitivity to haptens other than FITC, including a food ingredient PEITC. (C) 2010 Elsevier Ltd. All rights reserved.