Secretion and transport of mouse epididymal proteins after injection of 35S-methionine.
Secretion and transport of mouse epididymal proteins after injection of 35S-methionine.
复制标题
DOI：
10.1095/biolreprod43.1.113
复制
发表时间：
1990-07
影响因子：
3.6
通讯作者：
J. Vreeburg;M. Holland;G. Cornwall;M. Orgebin‐Crist
中科院分区：
文献类型：
--
作者：
J. Vreeburg;M. Holland;G. Cornwall;M. Orgebin‐Crist
本文观察了静脉注射后不同时间小鼠附睾腔液粗提液中附睾液分泌蛋白的存在。注射35S-蛋氨酸或附睾片与35S-蛋氨酸孵化后。体内注射后4、8、12和16h，腔蛋白的标记蛋白掺入量无显著差异，第1~3区(头部)粗液中的标记蛋白质量分别是第4区(体部)和第5区(尾部)的2倍和4倍。随着~(35)S-蛋氨酸剂量的增加，标记蛋白的量显著增加。附睾液中约有一半的标记蛋白存在于附睾液中。在注射同位素后8h或体外培养5h后，聚丙烯酰胺凝胶电泳法显示了相似的蛋白质图谱。然而，这五个区域的蛋白质图谱明显不同，具有很高的特征。体内注射后8h，在2区和3区的粗腔液中发现了2种蛋白质(25 kDa和18 kDa)，而在4区和5区中未检测到蛋白质。注射后8 h，在4区和5区中发现了高含量的蛋白质(29 KDa)。注射5天后，在第4和第5区发现了这三种蛋白质。然而，25 kDa的蛋白质含量较少，而18 kDa和29 kDa的蛋白质在尾液中积累。
The presence of epididymal secretory proteins in crude luminal fluids of the epididymis of mice was investigated at varying times after i.v. injection of 35S-methionine or after incubation of epididymal minces with 35S-methionine. The amount of label incorporated into luminal proteins after in vivo injection was not significantly different at 4, 8, 12, and 16 h. The quantity of labeled proteins in the crude luminal fluids of Regions 1-3 (caput) was about two and four times higher than in Regions 4 (corpus) and 5 (cauda), respectively. Increasing the dosage of 35S-methionine strongly increased the amount of labeled protein present. Approximately half of the labeled protein present in the epididymis was found in the luminal fluid. Polyacrylamide gel electrophoresis revealed comparable patterns of proteins at 8 h after injection of isotope or after a 5-h in vitro incubation of minced epididymal tissues with isotope. The protein patterns from the five regions, however, were markedly different from each other and highly characteristic. Two proteins (25 kDa and 18 kDa) were found in crude luminal fluids of Regions 2 and 3 eight hours after in vivo injection, but not in Regions 4 and 5. One protein (29 kDa) was found in high amounts in Regions 4 and 5 eight hours after injection. Five days after injection, the three proteins were found in Regions 4 and 5. However, the 25-kDa protein was present in reduced amount, whereas the 18- and 29-kDa proteins accumulated in caudal fluid.(ABSTRACT TRUNCATED AT 250 WORDS)