Development and evaluation of a blocking enzyme-linked immunosorbent assay for detection of avian metapneumovirus type C-specific antibodies in multiple domestic avian species
Development and evaluation of a blocking enzyme-linked immunosorbent assay for detection of avian metapneumovirus type C-specific antibodies in multiple domestic avian species
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DOI：
10.1128/jcm.41.8.3579-3583.2003
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发表时间：
2003-08-01
影响因子：
9.4
通讯作者：
Swayne, DE
中科院分区：
文献类型：
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作者：
Turpin, EA;Lauer, DC;Swayne, DE
1978年，南非描述了首例由禽偏肺病毒(AMPV)引起的感染火鸡的呼吸道疾病。病原体于1986年被分离并确认为肺炎病毒。在欧洲的家养非家禽物种中已经检测到aMPV，但在美国还没有检测这些病毒的测试。为了开始了解家鸭、鹅和野生水禽在AMPV流行病学中的潜在作用，我们建立并评价了一种检测AMPV C型(AMPV-C)特异性抗体的阻断酶联免疫吸附试验(BELISA)。这种检测方法克服了间接ELISA的物种特异性平台，可以检测潜在任何禽类的AMPV-C特异性抗体。用实验火鸡血清样本对Belisa进行了初步测试，结果发现与病毒中和试验和间接酶联免疫吸附试验(IEL ISA)的结果相一致。用该方法对明尼苏达州1000份火鸡群血清样品进行检测，结果与间接酶联免疫吸附试验的符合率为94.9%。此外，我们还能够证明，BELISA可以从实验感染的鸭子中检测到AMPV-C特异性抗体，这表明它在筛选多种禽类的血清样本方面是有用的。这是美国第一个检测多种禽类AMPV-C特异性抗体的诊断试验。
The first cases of infection caused by avian metapneumoviruses (aMPVs) were described in turkeys with respiratory disease in South Africa during 1978. The causative agent was isolated and identified as a pneumovirus in 1986. aMPVs have been detected in domestic nonpoultry species in Europe, but tests for the detection of these viruses are not available in the United States. To begin to understand the potential role of domestic ducks and geese and wild waterfowl in the epidemiology of aMPV, we have developed and evaluated a blocking enzyme-linked immunosorbent assay (bELISA) for the detection of aMPV type C (aMPV-C)-specific antibodies. This assay method overcomes the species-specific platform of indirect ELISAs to allow detection of aMPV-C-specific antibodies from potentially any avian species. The bELISA was initially tested with experimental turkey serum samples, and the results were found to correlate with those of virus neutralization assays and indirect enzyme-linked immunosorbent assay (iELISA). One thousand serum samples from turkey flocks in Minnesota were evaluated by our bELISA, and the level of agreement of the results of the bELISA and those of the iELISA was 94.9%. In addition, we were able to show that the bELISA could detect aMPV-C-specific antibodies from experimentally infected ducks, indicating its usefulness for the screening of serum samples from multiple avian species. This is the first diagnostic assay for the detection of aMPV-C-specific antibodies from multiple avian species in the United States.