Changes in gene expression involved in energy utilization during chicken follicle development
Changes in gene expression involved in energy utilization during chicken follicle development
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DOI：
10.1016/j.anireprosci.2005.09.016
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Akiba, Yukio
中科院分区：
文献类型：
--
作者：
Seol, H. S.;Sato, Kan;Akiba, Yukio
排卵前7天，颗粒细胞和卵泡膜细胞的DNA和蛋白质合成显著增加，此时卵泡发育迅速。然而，能量和底物来源，以促进广泛的DNA和蛋白质合成所需的卵泡尚未确定在鸟类物种。本研究旨在探讨从小白色卵泡（SAT）到卵泡I（F1）发育阶段卵泡膜中参与葡萄糖转运、糖酵解和脂肪酸氧化的调控基因的表达谱。在我们对葡萄糖转运蛋白（GLUT）亚型表达的分析中，GLUT 1 mRNA水平随卵泡发育而增加，而GLUT 2、GLUT 3和GLUT 8 mRNA水平不受卵泡发育的影响。与此相反，参与葡萄糖转运下游代谢的蛋白质，包括己糖激酶（HK），丙酮酸脱氢酶E1 α（PDH E I a）和柠檬酸合酶（CS）的表达模式并不随卵泡的发育阶段而变化，即使在快速卵泡生长期间。与脂肪酸β-氧化相关的基因（肉毒碱棕榈酰CoA转移酶I和II，L-3-羟酰CoA脱氢酶和长链酰基CoA脱氢酶）的表达，在哺乳动物物种的卵泡中的表达以前没有研究过，没有改变一致的卵泡发育。这些结果表明，葡萄糖和脂肪酸可能作为能量来源，以确保快速卵泡发育在鸡卵巢，即使糖酵解和β-氧化不受卵泡发育。(c)2005 Elsevier B.V.保留所有权利。
Ovarian follicle development in egg-laying species is characterized by rapid growth in 7 days prior to ovulation when DNA and protein synthesis is markedly increased in the granulosa and theca cells. However, energy and substrate sources to facilitate the extensive DNA and protein synthesis necessary for folliculogenesis have not been identified in avian species. The current study was undertaken to investigate the expression profiles of regulatory genes involved in glucose transport, glycolysis and fatty acid oxidation in the follicle membranes from the small white follicle (SAT) to follicle I (F1) stages of follicle development. In our analysis of glucose transporter (GLUT) isoform expression, the level of GLUT1 mRNA increased with follicle development while GLUT2, GLUT3 and GLUT8 mRNA levels were unaffected by follicle development. In contrast, the expression patterns of proteins involved in metabolism down-stream of glucose transport, including hexokinase (HK), pyruvate dehydrogenase E1 alpha (PDH E I a) and citrate synthase (CS), did not vary with the developmental stage of the follicle, even during rapid follicle growth. Expression of genes related to beta-oxidation of fatty acids (carnitine palmityl CoA transferase I and II, L-3-hydroxyacyl CoA dehydrogenase and long-chain acyl-CoA dehydrogenase), for which expression in the ovarian follicles of mammalian species has not previously been studied, was not changed consistently with the follicle development. These results suggest that both glucose and fatty acids might work as energy sources to ensure rapid follicle development in the chicken ovary, even though glycolysis and beta-oxidation are not modulated by follicle development. (c) 2005 Elsevier B.V. All rights reserved.