Resolution of sister telomere association is required for progression through mitosis
Resolution of sister telomere association is required for progression through mitosis
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DOI：
10.1126/science.1094754
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发表时间：
2004-04-02
期刊：
影响因子：
56.9
通讯作者：
Smith, S
中科院分区：
文献类型：
--
作者：
Dynek, JN;Smith, S
粘附素从姐妹染色单体在S期的复制开始到后期的开始，一直保持着姐妹染色单体的联系。在脊椎动物细胞中，有两条不同的途径使粘附素解离，一条作用于染色体臂，另一条作用于着丝粒。在这里，我们描述了作用于端粒的第三条途径。端粒多聚(ADP-核糖)聚合酶Tankyrase 1的敲除导致有丝分裂停止。染色体在中期平板上排列正常，但不能分离。姐妹染色单体在着丝粒和臂上分离，但在端粒上保持联系，显然是通过蛋白质桥连接的。因此，端粒可能需要一种独特的依赖于tankyrase 1的机制来在后期之前分辨姐妹染色单体。
Cohesins keep sister chromatids associated from the time of their replication in S phase until the onset of anaphase. In vertebrate cells, two distinct pathways dissociate cohesins, one acts on chromosome arms and the other on centromeres. Here, we describe a third pathway that acts on telomeres. Knockdown of tankyrase 1, a telomeric poly(ADP-ribose) polymerase caused mitotic arrest. Chromosomes aligned normally on the metaphase plate but were unable to segregate. Sister chromatids separated at centromeres and arms but remained associated at telomeres, apparently through proteinaceous bridges. Thus, telomeres may require a unique tankyrase 1-dependent mechanism for sister chromatid resolution before anaphase.