Sensitive, laser-assisted determination of complex oligosaccharide mixtures separated by capillary gel electrophoresis at high resolution.
Sensitive, laser-assisted determination of complex oligosaccharide mixtures separated by capillary gel electrophoresis at high resolution.
复制标题
对通过毛细管凝胶电泳分离的复杂寡糖混合物进行高分辨率激光辅助测定。
DOI：
10.1021/ac00032a026
复制
发表时间：
1992
影响因子：
7.4
通讯作者：
Novotny,MV
中科院分区：
文献类型：
--
作者：
Liu,J;Shirota,O;Novotny,MV
Scientific interest in complex carbohydrates has been rapidly increasing due to their multilateral biological roles in glycoproteins, proteoglycans, and various natural poly-saccharide forms. It is commonly perceived that substantial progress in this wide-ranging area of carbohydrate biochem-istry and physiology has been primarily hampered by the lack of effective analytical methodology for resolving complex mixtures, often available only at low sample concentrations. Traditional separation and measurement techniques, such as gas chromatography, gel filtration, or various forms of highperformance liquid chromatography, often lack an adequate resolving power to distinguish numerous oligomers and isom-eric species that may be present in a sample of interest. In addition, the general lack of detectable moieties within a typical carbohydrate structure further complicates the task of high-sensitivity measurements. 1 Although the scope of carbohydrate analysishas recently been enhanced by an ef-fective application of ion-exchange principles and pulsed amperometric detection, 2" 6 such improvement still falls short of the generalneeds in carbohydrate analysis. We report here the use of capillary gel electrophoresis with laser-induced fluorescence detection as a new and extremely powerful approach to the analysis of complex oligosaccharide mixtures. The virtues and potential of capillary electropho-resis (CE) have recently been explored with complex oligonucleotide mixtures, 7· 8 proteins (see ref 9 for a review), and a variety of small molecules. We have recently reported CE separations of the fluorescent derivatives of reducing neutral sugars10 and amino sugars11 in the open tubular format; however, the unfavorable mass-to-charge ratio of higher oli-gosaccharides prevented their effective resolution. This paper describes substantial improvements in this regard, made using a series of oligomers derived from polygalacturonic acid.
