Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques
Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques
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DOI：
10.1099/vir.0.18808-0
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Barry, PA
中科院分区：
文献类型：
--
作者：
Huff, JL;Eberle, R;Barry, PA
非人灵长类疱疹病毒在没有疾病临床体征的情况下在免疫活性宿主中建立并维持终身持续感染。理解非人灵长类疱疹病毒自然史的一个基本问题是病毒是否保持在真正的潜伏状态或以低水平慢性表达为特征的状态。为了解决这个问题，实时PCR检测定量猕猴粘膜液中的1型猕猴疱疹病毒（B病毒）DNA。该检测方法快速、灵敏（110个基因组拷贝），对从多种猕猴中获得的B病毒具有特异性。将B病毒的散毒特征与另一种地方性疱疹病毒恒河猴巨细胞病毒（RhCMV）在群体饲养猴中进行比较。每天从两组血清阳性猴中采集粘液拭子或唾液样本，这两组猴经历了压力性搬迁（第1组）或日常椅子约束（第2组）。在繁殖季节搬迁的4只动物（第1组）的粘液中检测到了B病毒DNA，但在一年中其他时间搬迁的10只动物的粘液中未检测到B病毒DNA。在任何第2组猴中均未检出B病毒DNA。相比之下，在第1组和第2组的大多数动物中均检测到RhCMV DNA。检测到B病毒DNA脱落是与繁殖季节相关的相对罕见的事件，而RhCMV DNA在大多数猴的粘膜液中持续检测到。
Non-human primate herpesviruses establish and maintain a lifelong persistent infection in immunocompetent hosts in the absence of clinical signs of disease. A fundamental issue for understanding the natural history of non-human primate herpesviruses is whether the viruses are maintained in a truly latent state or one characterized by a low level of chronic expression. To address this issue, a real-time PCR assay was developed to quantify Cercopithecine herpesvirus type 1 (B virus) DNA in mucosal fluids of rhesus macaques. This assay was rapid, sensitive (110 genome copies) and specific for B virus obtained from multiple species of macaques. The shedding profile of B virus was compared to another endemic herpesvirus, rhesus; cytomegalovirus (RhCMV), in colony-reared monkeys. Mucosal swabs or saliva samples were taken daily from two groups of seropositive monkeys undergoing either a stressful relocation (group 1) or daily chair restraint (group 2). B virus DNA was detected in mucosal fluids from four animals relocated during the breeding season (group 1) but not from 10 animals moved at other times of the year. No B virus DNA was detected in any group 2 monkey. In contrast, RhCMV DNA was detected in the majority of animals of both groups 1 and 2. Detection of B virus DNA shedding is a relatively rare event associated with the breeding season, while RhCMV DNA is persistently detected in mucosal fluids of most monkeys.