Triazole Fungicides Inhibit Zebrafish Hatching by Blocking the Secretory Function of Hatching Gland Cells.
Triazole Fungicides Inhibit Zebrafish Hatching by Blocking the Secretory Function of Hatching Gland Cells.
复制标题
DOI：
10.3390/ijms18040710
复制
发表时间：
2017-04-04
影响因子：
5.6
通讯作者：
Allende ML
中科院分区：
文献类型：
--
作者：
De la Paz JF;Beiza N;Paredes-Zúñiga S;Hoare MS;Allende ML
在动物中，孵化代表着从发育中的胚胎到自由生活的个体（幼虫）的过渡点。该过程受到许多内源性和环境因素的精细调节，并且已被证明对多种化学试剂敏感。通常在生物测定中对其进行评估，以确定不同试剂对鱼类和其他水生动物早期发育和繁殖能力的影响。在鱼类中，绒毛膜的分解是通过孵化腺细胞 (HGC) 分泌绒毛膜溶解素到卵周间隙 (PVS) 以及发育中的幼虫的自发运动来实现的。在这项工作中，我们使用斑马鱼来分析一系列广泛使用的农用化学品——三唑酮三唑酮 (FON)、三唑酮 (NOL) 和游离三唑 (1,2,4-T) 对孵化成功的影响。我们发现三唑暴露对孵化有强烈的抑制作用，这与 HGC 的形态变化和分泌功能的降低有关。结果，HGC 释放的绒毛膜溶解酶减少。我们还发现，暴露于 FON 后 HGC 分泌减少可以通过与多巴胺 D2 受体拮抗剂共孵育来挽救，但不能通过 D1 样受体拮抗剂来挽救。这表明该系列杀菌剂可能通过特定途径损害鱼类生命周期中的关键事件。
In animals, hatching represents the transition point from a developing embryo to a free-living individual, the larva. This process is finely regulated by many endogenous and environmental factors and has been shown to be sensitive to a variety of chemical agents. It is commonly evaluated in bioassays in order to establish the effects of different agents on early development and reproductive capabilities in fish and other aquatic animals. In fish, the breakdown of the chorion is achieved by the secretion of choriolysin by hatching gland cells (HGCs) into the perivitelline space (PVS), coupled with spontaneous movements of the developing larva. In this work, we used zebrafish to assay the effects of a family of widely used agrochemicals—triazoles Triadimefon (FON), Triadimenol (NOL) and free triazole (1,2,4-T)—on hatching success. We found a strong inhibition of hatching by triazole exposure which was correlated with morphological changes and a reduction in the secretory function of the HGCs. As a consequence, the release of choriolytic enzymes by HGCs was reduced. We also found that HGC secretion reduction after exposure to FON can be rescued by co-incubation with a dopamine D2 receptor antagonist but not by antagonists of the D1-like receptors. This suggests a specific pathway through which this family of fungicides may be impairing a critical event in the fish life cycle.