Development of first linkage map for Silphium integrifolium (Asteraceae) enables identification of sporophytic self-incompatibility locus
Development of first linkage map for Silphium integrifolium (Asteraceae) enables identification of sporophytic self-incompatibility locus
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DOI：
10.1038/s41437-022-00530-4
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发表时间：
2022-04-18
期刊：
影响因子：
3.8
通讯作者：
Smith, Kevin P.
中科院分区：
文献类型：
--
作者：
Price, John H.;Raduski, Andrew R.;Smith, Kevin P.
全缘叶松香草（菊科）已被确定为一个候选人的驯化作为一个多年生油料作物，并被认为具有孢子体自交不亲和系统，其遗传基础是不是很清楚，在菊科。为了解决这一差距，我们试图绘制该物种自我识别位点（S-位点）的基因组位置。我们使用双亲群体和测序基因分型来创建该物种的第一个遗传连锁图谱，其中包含198个SNP标记并解析为正确数量的连锁群。然后，我们开发了一种新的杂交方案和一套分析方法，以推断这些个体的一个子集的S-位点基因型，使我们能够绘制性状。最后，我们评估了潜在的感兴趣的基因，使用同线性分析与一年生向日葵（向日葵）和莴苣（莴苣）基因组。我们的结果证实S.全缘叶确实具有孢子体自交不亲和系统。我们的方法是有效和高效的，使我们能够映射的S。与现有方法相比，全缘叶S-位点的方法使用更少的资源，并且可以容易地应用于其他物种。
Silphium integrifolium (Asteraceae) has been identified as a candidate for domestication as a perennial oilseed crop and is assumed to have sporophytic self-incompatibility system-the genetic basis of which is not well understood in the Asteraceae. To address this gap, we sought to map the genomic location of the self-recognition locus (S-locus) in this species. We used a biparental population and genotyping-by-sequencing to create the first genetic linkage map for this species, which contained 198 SNP markers and resolved into the correct number of linkage groups. Then we developed a novel crossing scheme and set of analysis methods in order to infer S-locus genotypes for a subset of these individuals, allowing us to map the trait. Finally, we evaluated potential genes of interest using synteny analysis with the annual sunflower (Helianthus annuus) and lettuce (Lactuca sativa) genomes. Our results confirm that S. integrifolium does indeed have a sporophytic self-incompatibility system. Our method is effective and efficient, allowed us to map the S. integrifolium S-locus using fewer resources than existing methods, and could be readily applied to other species.