Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development
Newborn horizontal cells migrate bi-directionally across the neuroepithelium during retinal development
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DOI：
10.1242/dev.01018
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发表时间：
2004-03-01
期刊：
影响因子：
4.6
通讯作者：
Hallböök, F
中科院分区：
文献类型：
--
作者：
Edqvist, PHD;Hallböök, F
细胞迁移在视网膜发展过程中起着重要作用。在这项工作中，我们研究了禽类视网膜中新生儿水平细胞的迁移。使用早期表达的转录因子Lim1和Prox1的模式，我们表明水平细胞从他们的出生部位迁移，靠近心室（玻璃体）的神经上皮的侧面（玻璃体），接下来它们就在那儿对齐到前瞻性神经节细胞层，然后再次迁移回内部核层外部的最终层流位置。迁移发生在汉堡和汉密尔顿阶段24和33之间，相当于胚胎第4.5天和第8天。在第26阶段至30阶段，水平细胞位于神经节细胞层和内部。在第29阶段，抑制迁移的肌动蛋白聚合阻滞剂细胞链蛋白酶D的眼部注射会干扰水平细胞向其最终目的地的迁移。此外，使用视网膜切片的绿色荧光蛋白表达载体使用生物学基因转移，我们能够通过延时显微镜记录心室导向的迁移。与免疫组织化学相结合，我们表明，也已经在心室方向易位的转染细胞对水平细胞标记LIM1和PROX1呈阳性。这项工作中描述的替代迁移路径与普遍接受的水平细胞不同，并且该知识将影响如何指定水平细胞的分子测定。
Cell migration plays an important role during the development of the retina. In this work we have studied the migration of newborn horizontal cells in avian embryonic retina. Using the pattern of the early expressed transcription factors Lim1 and Prox1 we have shown that horizontal cells migrate bi-directionally from their site of birth, close to the ventricular side, to the adjacent (vitreal) side of the neuroepithelium, where they align just next to the prospective ganglion cell layer before migrating back again to their final laminar position in the external part of the inner nuclear layer. The migration occurs between Hamburger and Hamilton stages 24 and 33, which is equivalent to embryonic day 4.5 and 8. Between stages 26 and 30 the horizontal cells reside close to the ganglion cell layer and intra. ocular injections of a cytochalasin D, an actin polymerisation blocker that inhibit migration, at stage 29 interfered with the migration of the horizontal cells to their final destination. Furthermore, using biolistic gene transfer with a green fluorescence protein expression vector of retinal slices we were able to record ventricle-directed migration by time-lapse microscopy. Combining biolistics with immunohistochemistry we showed that transfected cells, which have also been translocated in a ventricular direction were positive for the horizontal cell markers Lim1 and Prox1. The alternative path of migration that is described in this work differs from the generally accepted one for horizontal cells and this knowledge will influence the view of how the molecular determination of horizontal cells is specified.