Crystallization and preliminary X-ray crystallographic analysisof UDP-glucuronic acid :flavonol-3-O-glucuronosyltransferase
Crystallization and preliminary X-ray crystallographic analysisof UDP-glucuronic acid :flavonol-3-O-glucuronosyltransferase
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UDP-葡萄糖醛酸：黄酮醇-3-O-葡萄糖醛酸基转移酶的结晶和初步X射线晶体分析
DOI：
10.1107/s1744309112045095
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Mizohata;E.;Okuda;T.;Hatanaka;S.;Nakayama;T.;Horikawa;M.;Nakayama;T.;Ono;E.;and Inoue;T.
葡萄藤（葡萄属vinifera）糖基转移酶5（VvGT 5）是UDP-葡萄糖醛酸：黄酮醇-3-O-葡萄糖醛酸基转移酶，其催化使用UDP-葡萄糖醛酸作为糖供体的黄酮醇的3-O-特异性葡萄糖醛酸基化以产生黄酮醇3-O-葡萄糖苷，其是重要的生物活性植物化学物质。通过坐滴气相扩散法纯化并结晶在大肠杆菌细胞中表达的重组VvGT 5。使用同步辐射源从单晶收集到2.2 μ m布拉格间距的全套X射线衍射数据。晶体为六方晶系，空间群为P6122，晶胞参数a = B = 102.70，c = 535.92 nm。 用分子置换法确定了初始相。
Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase that catalyses the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting-drop vapour-diffusion method. A full set of X-ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron-radiation source. The crystal was hexagonal, belonging to space group P6122, with unit-cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular-replacement method.