Generation of induced pluripotent stem cell line (XDCMHi001-A) from an Ankylosing spondylitis patient with JAK2 mutation
Generation of induced pluripotent stem cell line (XDCMHi001-A) from an Ankylosing spondylitis patient with JAK2 mutation
复制标题
从具有 JAK2 突变的强直性脊柱炎患者中产生诱导多能干细胞系 (XDCMHi001-A)
DOI：
10.1016/j.scr.2020.101788
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发表时间：
2020-05-01
影响因子：
1.2
通讯作者：
Quan, Renfu
中科院分区：
文献类型：
--
作者：
Hu, Jintao;Ren, Weifan;Quan, Renfu
遗传是导致强直性脊柱炎（AS）易感性的主要因素。Janus激酶2（JAK 2）与AS相关。使用来自具有JAK 2突变的AS患者的尿来源的细胞来产生具有五种附加型iPSC重编程载体（pCXLE-hOCT 3/4-shp 53-F、pCXLE-hSK、pCXLE-hUL、pCXLE-EGFP和pCXWB-EBNA 1）的诱导多能干细胞（iPSC）。诱导的iPSCs具有多能性，为研究JAK 2在AS发病中的作用及机制提供了实验依据。
Heredity is the major factor contributing to the susceptibility to ankylosing spondylitis(AS). Janus kinase 2 ( JAK2 ) has been associated with AS. Urine-derived cells from an AS patient with JAK2 mutation were used to generate induced pluripotent stem cells (iPSCs) with five episomal iPSC reprogramming vectors (pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXLE-EGFP and pCXWB-EBNA1). The iPSCs were pluripotent and will be valuable for research on the role and mechanism of JAK2 in the pathogenesis of AS.