The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.

The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
复制标题

酵母双杂交系统检测枯草芽孢杆菌 sigmaB 调节剂之间的相互作用。

DOI:
10.1128/jb.178.23.7020-7023.1996
复制
发表时间:
1996
影响因子:
3.2
通讯作者:
Haldenwang,WG
Haldenwang,WG
中科院分区:
生物学3区
文献类型:
--
作者:
Voelker,U;Voelker,A;Haldenwang,WG

文献摘要

参考文献

相似文献

SigmaB是枯草芽孢杆菌的一般应激反应σ因子,由与其共转录的七个基因(rsbR、S、T、U、V、W和X)的产物调节。生物化学技术以前揭示了RsbW,RsbV和sigmaB之间的物理关联,但未能检测到RsbR,S,T,U或X彼此或RsbV,RsbW或sigmaB的相互作用。使用酵母双杂交系统,我们现在已经获得了这种相互作用的证据。当RsbS与RsbR或RsbT配对,RsbR与RsbT配对,RsbV与RsbU或RsbW配对时,酵母报告系统被激活。此外,检测到RsbW2和RsbR2二聚体形成。RsbX未能显示与自身或任何其他sigB操纵子产物的相互作用。
SigmaB, the general stress response sigma factor of Bacillus subtilis, is regulated by the products of seven genes (rsbR, S, T, U, V, W, and X) with which it is cotranscribed. Biochemical techniques previously revealed physical associations among RsbW, RsbV, and sigmaB but failed to detect interactions of RsbR, S, T, U, or X with each other or RsbV, RsbW, or sigmaB. Using the yeast two-hybrid system, we have now obtained evidence for such interactions. The yeast reporter system was activated when RsbS was paired with either RsbR or RsbT, RsbR was paired with RsbT, and RsbV was paired with either RsbU or RsbW. In addition, RsbW2 and RsbR2 dimer formation was detected. RsbX failed to show interactions with itself or any of the other sigB operon products.
“1972政权”日本安保体系下的台湾(中文)
DOI: --
发表时间: 2022
期刊:
影响因子: --
作者:
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国:EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者: 福田円
酵母遗传学方法:实验室手册
DOI: 10.1016/0378-1119(87)90435-5
发表时间: 1981
期刊: Cold Spring Harbor Monograph Archive
影响因子: --
作者:
L. Sherman;G. Fink;J. Hicks
通讯作者: J. Hicks
DOI: 10.1006/jmbi.1996.0390
发表时间: 1996-07-12
影响因子: 5.6
作者:
Alper, S;Dufour, A;Losick, R
通讯作者: Losick, R
DOI: 10.1073/pnas.90.6.2330
发表时间: 1993-03-15
影响因子: 11.1
作者:
BENSON, AK;HALDENWANG, WG
通讯作者: HALDENWANG, WG
SpoIIE 在枯草芽孢杆菌孢子形成过程中控制转录因子 oM 蛋白的磷酸化状态
DOI: --
发表时间: 2005
期刊:
影响因子: --
作者:
Fabrizio;Arigoni;Leonard;DUNCANt;Scott ALPERt;Richard LOSICKt;P. Stragier
通讯作者: P. Stragier