The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
复制标题
酵母双杂交系统检测枯草芽孢杆菌 sigmaB 调节剂之间的相互作用。
DOI:
10.1128/jb.178.23.7020-7023.1996
复制
发表时间:
1996
影响因子:
3.2
通讯作者:
Haldenwang,WG
中科院分区:
文献类型:
--
作者:
Voelker,U;Voelker,A;Haldenwang,WG
SigmaB, the general stress response sigma factor of Bacillus subtilis, is regulated by the products of seven genes (rsbR, S, T, U, V, W, and X) with which it is cotranscribed. Biochemical techniques previously revealed physical associations among RsbW, RsbV, and sigmaB but failed to detect interactions of RsbR, S, T, U, or X with each other or RsbV, RsbW, or sigmaB. Using the yeast two-hybrid system, we have now obtained evidence for such interactions. The yeast reporter system was activated when RsbS was paired with either RsbR or RsbT, RsbR was paired with RsbT, and RsbV was paired with either RsbU or RsbW. In addition, RsbW2 and RsbR2 dimer formation was detected. RsbX failed to show interactions with itself or any of the other sigB operon products.
登录
查看更多内容
DOI:
--
发表时间:
2022
期刊:
影响因子:
--
作者:
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国:EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者:
福田円
DOI:
10.1016/0378-1119(87)90435-5
发表时间:
1981
期刊:
Cold Spring Harbor Monograph Archive
影响因子:
--
作者:
L. Sherman;G. Fink;J. Hicks
通讯作者:
J. Hicks
影响因子:
5.6
作者:
Alper, S;Dufour, A;Losick, R
通讯作者:
Losick, R
DOI:
10.1073/pnas.90.6.2330
发表时间:
1993-03-15
影响因子:
11.1
作者:
BENSON, AK;HALDENWANG, WG
通讯作者:
HALDENWANG, WG
DOI:
--
发表时间:
2005
期刊:
影响因子:
--
作者:
Fabrizio;Arigoni;Leonard;DUNCANt;Scott ALPERt;Richard LOSICKt;P. Stragier
通讯作者:
P. Stragier