A maize calcineurin B-like interacting protein kinase ZmCIPK42 confers salt stress tolerance
A maize calcineurin B-like interacting protein kinase ZmCIPK42 confers salt stress tolerance
复制标题
玉米钙调神经磷酸酶 B 样相互作用蛋白激酶 ZmCIPK42 赋予盐胁迫耐受性
DOI：
10.1111/ppl.13244
复制
发表时间：
2020-11-08
影响因子：
6.4
通讯作者：
Huang, Quansheng
中科院分区：
文献类型：
--
作者：
Chen, Xunji;Chen, Guo;Huang, Quansheng
类钙调磷酸酶B（CBL）和CBL相互作用蛋白激酶（CIPK）在生物和非生物胁迫反应中起着重要作用。然而，不同的CIPKs在生物和非生物胁迫反应中的作用还不太清楚。本研究通过查询玉米B73 EMS突变体库，从玉米中寻找与盐胁迫耐受性和离子稳态相关的基因，发现了导致玉米CIPK基因ZmCIPK42编码区发生G → A突变的早期蛋白质终止突变。突变体zmcipk42植株在苗期具有较少的分枝雄穗和受损的盐胁迫耐受性。实时荧光定量PCR分析表明，ZmCIPK42在不同组织中均有表达，且受NaCl胁迫诱导。酵母双杂交筛选鉴定了蛋白酶抑制剂（ZmMPI）以及钙调磷酸酶B样蛋白1和蛋白4（ZmCBL1、ZmCBL4）作为ZmCIPK42的相互作用伴侣。这些相互作用进一步证实了在植物细胞中的双分子荧光互补。此外，过表达ZmCIPK42导致玉米和拟南芥对高盐的耐受性增强。这些结果表明，ZmCIPK42是一个盐胁迫耐受性的正调控因子，是一个有希望的候选基因，通过遗传操作来提高玉米的耐盐性。
Calcineurin B-like (CBL) and CBL-interacting protein kinase (CIPK) play a crucial role in biotic and abiotic stress responses. However, the roles of different CIPKs in biotic and abiotic stress responses are less well characterized. In this study, we identified a mutation leading to an early protein termination of the maize CIPK gene ZmCIPK42 that undergoes a G to A mutation at the coding region via searching for genes involved in salt stress tolerance and ion homeostasis from maize with querying the EMS mutant library of maize B73. The mutant zmcipk42 plants have less branched tassel and impaired salt stress tolerance at the seedling stage. Quantitative real-time PCR analysis revealed that ZmCIPK42was expressed in diverse tissues and was induced by NaCl stress. A yeast two-hybrid screen identified a proteinase inhibitor (ZmMPI) as well as calcineurin B-like protein 1 and protein 4 (ZmCBL1, ZmCBL4) as interaction partners of ZmCIPK42. These interactions were further confirmed by bimolecular fluorescence complementation in plant cells. Moreover, over-expressing ZmCIPK42 resulted in enhanced tolerance to high salinity in both maize and Arabidopsis. These findings suggest that ZmCIPK42 is a positive regulator of salt stress tolerance and is a promising candidate gene to improve salt stress tolerance in maize through genetic manipulation.