SEQUENCE PERIODICITIES IN CHICKEN NUCLEOSOME CORE DNA
SEQUENCE PERIODICITIES IN CHICKEN NUCLEOSOME CORE DNA
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DOI：
10.1016/0022-2836(86)90452-3
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发表时间：
1986-10-20
影响因子：
5.6
通讯作者：
TRAVERS, AA
中科院分区：
文献类型：
--
作者：
SATCHWELL, SC;DREW, HR;TRAVERS, AA
DNA 围绕组蛋白八聚体的旋转定位似乎是由 DNA 结构的某些序列依赖性调节决定的。为了确定这些相互作用的详细性质，我们分析了来自鸡红细胞核心颗粒的 177 种不同 DNA 分子的序列。这些分子序列内容的所有变化（可能归因于 DNA 弯曲的序列依赖性偏好）与 DNA 包裹核小体核心晶体结构中组蛋白八聚体时的详细路径密切相关。与 DNA 相对于蛋白质表面的旋转方向最密切相关的序列依赖性偏好有两种：ApApA/TpTpT 和 ApApT/ApTpT，其小凹槽主要面向蛋白质；还有 GpGpC/GpCpC 和 ApGpC/GpCpT，其小凹槽朝外。傅里叶分析已用于获得所有 10 个二核苷酸和所有 32 个三核苷酸排列的出现分数变化。这些序列偏好应该普遍适用于蛋白质-DNA 识别的许多其他情况，其中 DNA 包裹着蛋白质。此外，还观察到均聚物 (dA) .cntdot 的长期运行。 (dT) 更喜欢占据核心 DNA 的末端，距离二元组五到六圈。这些相同的序列显然被排除在核心 DNA 的近中心之外，距离二元体两到三圈。因此，任何单个组蛋白八聚体沿着确定序列的 DNA 分子的翻译定位可能会受到 (dA).cntdot 位置的强烈影响。 (dT) 序列。它还可能受到蛋白质对“接头”区域中序列的任何厌恶的影响，该区域的序列内容仍有待确定。
The rotational positioning of DNA about the histone octamer appears to be determined by certain sequence-dependent modulations of DNA structure. To establish the detailed nature of these interactions, we have analysed the sequences of 177 different DNA molecules from chicken erythrocyte core particles. All variations in the sequence content of these molecules, which may be attributed to sequence-dependent preferences for DNA bending, correlate well with the detailed path of the DNA as it wraps around the histone octamer in the crystal structure of the nucleosome core. The sequence-dependent preferences that correlate most closely with the rotational orientation of the DNA, relative to the surface of the protein, are of two kinds: ApApA/TpTpT and ApApT/ApTpT, the minor grooves of which face predominantly in towards the protein; and also GpGpC/GpCpC and ApGpC/GpCpT, whose minor grooves face outward. Fourier analysis has been used to obtain fractional variations in occurrence for all ten dinucleotide and all 32 trinucleotide arrangements. These sequence preferences should apply generally to many other cases of protein-DNA recognition, where the DNA wraps around a protein. In addition, it is observed that long runs of homopolymer (dA) .cntdot. (dT) prefer to occupy the ends of core DNA, five to six turns away from the dyad. These same sequences are apparently excluded from the near-centre of core DNA, two to three turns from the dyad. Hence, the translational positioning of any single histone octamer along a DNA molecule of defined sequence may be strongly influenced by the placement of (dA) .cntdot. (dT) sequences. It may also be influenced by any aversion of the protein for sequences in the "linker" region, the sequence content of which remains to be determined.