HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root
HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root
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DOI：
10.1016/j.jchromb.2007.11.041
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Wang, Xiaoru
中科院分区：
文献类型：
--
作者：
Han, Chao;Shen, Yan;Wang, Xiaoru
建立了太子参药材指纹图谱的高效液相色谱分析方法。帕克斯。首次采用LC-TOF-MS联用技术对指纹图谱的成分进行了鉴定。用在线LC-TOF-MS分析了12个主要的指纹图谱峰，明确鉴定了1个环肽，并根据MS数据对5个环肽进行了初步归属。这些环肽作为高效液相指纹图谱中的标志峰。通过相似度计算和层次聚类分析对指纹图谱进行了分析。结果表明，利用高效液相指纹图谱可以确定异叶松的最佳采收期。并对该草本植物的种类进行鉴定。(C)2007 Elsevier B.V.保留所有权利。
High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (LC-TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC-TOF-MS analysis; one cyclic peptide was unequivocally identified and five cyclic peptides were tentatively assigned based on their MS data. These cyclic peptides served as the marker peaks in the HPLC fingerprints. The chromatographic fingerprints have been analyzed by similarity index calculations and hierarchical clustering analysis (HCA). The result showed that the HPLC fingerprints could be used to determine the optimal harvest time for R heterophylla (Miq.) Pax and to authenticate the species of the herb. (c) 2007 Elsevier B.V. All rights reserved.