Characterization of 13 polymorphic microsatellite loci in the Japanese land leech
Characterization of 13 polymorphic microsatellite loci in the Japanese land leech
复制标题
日本水蛭 13 个多态性微卫星位点的特征
DOI：
10.1016/j.parint.2017.10.002
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发表时间：
2018
影响因子：
1.9
通讯作者：
Aizawa M.
中科院分区：
文献类型：
--
作者：
Morishima K.;Suzuki T.;Aizawa M.
利用Illumina MiSeq测序方法，对日本水蛭（Haemadipsa japonica; Haemadipsidea）进行了13个多态微卫星位点的测序。共筛选了42064个核DNA contigs进行微卫星基序筛选，鉴定出30873个简单序列重复位点。从这些序列中，我们选择了30个引物集，其中13个位点被成功扩增。对这13个基因座的多态性进行了测试，使用了来自日本16个种群的16个样本。等位基因数和多态性信息含量分别在5 ~ 17和0.335 ~ 0.883之间变化，观察杂合度和期望杂合度分别在0.143 ~ 0.875和0.349 ~ 0.893之间变化，表明这些位点具有多态性。此外，我们利用这些基因座建立了有用的多重PCR。本文所描述的13个微卫星位点是陆地水蛭种的首个核微卫星标记。
We developed 13 polymorphic microsatellite loci of the Japanese land leech (Haemadipsa japonica; Haemadipsidea) using an Illumina MiSeq sequencing approach. A total of 42,064 nuclear DNA contigs were filtered for microsatellite motifs, among which 30,873 simple sequence repeat loci were identified. From these sequences, we selected 30 primer sets, and 13 of these loci were successfully amplified. Polymorphism of the 13 loci was tested using 16 individuals sampled from sixteen populations across Japan. The number of alleles and polymorphism information content varied from 5 to 17 and 0.335 to 0.883, respectively, and observed and expected heterozygosity values ranged from 0.143 to 0.875 and 0.349 to 0.893, respectively, indicating that these loci are polymorphic. Furthermore, we established useful multiplex PCR using these loci. The 13 microsatellite loci described in this paper are the first nuclear microsatellite markers for a land leech species.