Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase.
Expression of a gibberellin-induced leucine-rich repeat receptor-like protein kinase in deepwater rice and its interaction with kinase-associated protein phosphatase.
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DOI：
10.1104/pp.120.2.559
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发表时间：
1999-06
期刊：
影响因子：
7.4
通讯作者：
E. Knaap;Wen-Yuan Song;Deling Ruan;Margret Sauter;Pamela C. Ronald;Hans Kende
中科院分区：
文献类型：
--
作者：
E. Knaap;Wen-Yuan Song;Deling Ruan;Margret Sauter;Pamela C. Ronald;Hans Kende
我们在深水水稻(Oryza sativa L.)一种编码富含亮氨酸重复序列的类似受体的跨膜蛋白激酶的基因，OsTMK(水稻跨膜蛋白激酶)。水稻节间OsTMK基因的转录水平随赤霉素浓度的升高而增加。OsTMK在细胞分裂和伸长区域的表达尤其高。OsTMK的激酶结构域具有酶活性，可在丝氨酸和苏氨酸残基上自动磷酸化。克隆了水稻2C型蛋白磷酸酶(OsKAPP)的同源基因。KAPPS是激酶介导的信号转导通路中可能的下游成分。在体外，OsKAPP的激酶相互作用结构域被OsTMK的激活域所磷酸化。RNA凝胶印迹分析表明，OsTMK和OsKAPP在水稻植株不同组织中的表达相似。在蛋白结合分析中，OsKAPP与拟南芥的受体样蛋白激酶RLK5相互作用，但不与水稻和玉米受体样蛋白激酶Xa21和ZmPK1的蛋白激酶结构域相互作用。
We identified in deepwater rice (Oryza sativa L.) a gene encoding a leucine-rich repeat receptor-like transmembrane protein kinase, OsTMK (O. sativa transmembrane kinase). The transcript levels of OsTMK increased in the rice internode in response to gibberellin. Expression of OsTMK was especially high in regions undergoing cell division and elongation. The kinase domain of OsTMK was enzymatically active, autophosphorylating on serine and threonine residues. A cDNA encoding a rice ortholog of a kinase-associated type 2C protein phosphatase (OsKAPP) was cloned. KAPPs are putative downstream components in kinase-mediated signal transduction pathways. The kinase interaction domain of OsKAPP was phosphorylated in vitro by the kinase domain of OsTMK. RNA gel-blot analysis indicated that the expression of OsTMK and OsKAPP was similar in different tissues of the rice plant. In protein-binding assays, OsKAPP interacted with a receptor-like protein kinase, RLK5 of Arabidopsis, but not with the protein kinase domains of the rice and maize receptor-like protein kinases Xa21 and ZmPK1, respectively.