Shorter telomeres in adults with Type 1 diabetes correlate with diabetes duration, but only weakly with vascular function and risk factors
Shorter telomeres in adults with Type 1 diabetes correlate with diabetes duration, but only weakly with vascular function and risk factors
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DOI：
10.1016/j.diabres.2016.04.040
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发表时间：
2016-07-01
影响因子：
5.1
通讯作者：
Jenkins, Alicia J.
中科院分区：
文献类型：
--
作者：
Januszewski, Andrzej S.;Sutanto, Surya S.;Jenkins, Alicia J.
目的：探讨1型糖尿病(T1D)患者外周血白细胞(WBC)端粒长度是否比非糖尿病患者(Non-DB)短，并探讨其与血管生物标志物的关系。研究设计与方法：对140例非糖尿病患者和199例T1D患者，其中128例无血管并发症(T1DNoCx)和71例有血管并发症(T1DCx)，采用定量聚合酶链式反应(QRT)测定WBC相对端粒长度(RTL)。分析年龄、T1D病程、动脉弹性、脉压和血管危险因素与RTL的关系。年龄调整后的RTL在T1D组较非DB组短(1.48+/-0.03AU比1.64+/-0.04AU，p=0.002)，但与T1D并发症状态(T1DNoCX1.50+/-0.04vs.T1DCX1.46+/-0.05，p=0.5)无关，也与动脉弹性无关。单因素相关分析显示，RTL与年龄r=-0.27，p=0.0001，持续时间r=-0.16，p=0.03，脉压(r=-0.15，p=0.04)呈负相关，而与糖化血红蛋白、血压、肾功能(血肌酐、肌酐、肾功能)、血脂、胰岛素敏感性、炎症(C反应蛋白、钙调蛋白)或氧化应激(氧化低密度脂蛋白、氧化低密度脂蛋白/低密度脂蛋白-C、髓磷脂、PON-1)无关。多元回归分析显示，年龄和存在T1D是影响RTL的独立因素(r=0.29p<；0.0001)。结论：在本次横断面研究中，T1D的端粒较短。RTL与T1D病程呈负相关，但与并发症状态无关，与脉压和血管危险因素相关性弱。只有年龄和T1D是RTL的独立决定因素。值得进行纵向研究。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: To determine if white blood cell (WBC) telomeres are shorter in Type 1 diabetes (T1D) than in subjects without diabetes (non-DB), and shorter in T1D subjects with vs. without vascular complications; and to determine associations with vascular biomarkers.Research design and methods: WBC relative telomere length (RTL) was determined by quantitative PCR in a cross-sectional study of 140 non-DB and 199 T1D adults, including 128 subjects without vascular complications (T1DNoCx) and 71 subjects with vascular complications (T1DCx). Relationships of RTL with age, T1D duration, arterial elasticity, pulse pressure and vascular risk factors were determined.Results: RTL did not differ by gender within T1D and non-DB groups. Age-adjusted RTL was shorter in T1D vs. non-DB subjects (1.48 +/- 0.03 AU vs. 1.64 +/- 0.04 AU, p = 0.002), but did not differ by T1D complication status (T1DNoCX 1.50 +/- 0.04 vs. T1DCX 1.46 +/- 0.05, p = 0.50), nor correlate with arterial elasticity. Univariate analysis in T1D showed RTL correlated (inversely) with age r = -0.27, p = 0.0001, T1D duration r = -0.16, p = 0.03, and pulse pressure (r = -0.15, p = 0.04), but not with HbA1c, BP, renal function (serum creatinine, ACR, eGFR), lipids, insulin sensitivity, inflammation (CRP, CAMs) or oxidative stress (OxLDL, OxLDL/LDL-C, MPO, PON-1). Multiple regression analysis showed independent determinants of RTL were age and T1D presence (r = 0.29, p < 0.0001).Conclusions: In this cross-sectional study telomeres were shorter in T1D. RTL correlated inversely with T1D duration, but did not differ by complication status and weakly correlated with pulse pressure and vascular risk factors. Only age and T1D were independent determinants of RTL. Longitudinal studies are merited. (C) 2016 Elsevier Ireland Ltd. All rights reserved.