Effect of aphidicolin on viral and human DNA polymerases.
Effect of aphidicolin on viral and human DNA polymerases.
复制标题
阿菲迪霉素对病毒和人类 DNA 聚合酶的影响。
DOI：
10.1016/0006-291x(79)91106-9
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发表时间：
1979
影响因子：
3.1
通讯作者：
S. Spadari
中科院分区：
文献类型：
--
作者：
G. Pedrali;S. Spadari
单纯性疱疹病毒和牛痘病毒诱导的DNA聚合酶被阿霉素抑制，这种抑制可能是其体内抗病毒活性的基础。然而，其可能的临床应用受到其对人类复制DNA聚合酶α的伴随作用的阻碍。人α-聚合酶的抑制作用在体内和体外都是可逆的，而胸苷与DNA结合率的变化，在阿菲迪克林处理一代时间后，表明细胞可能因该药物而同步。DNA聚合酶β (DNA polymerase β)是一种对受损细胞核DNA进行修复合成的酶，在体内和体外均不受阿霉素的抑制，这表明阿霉素可以快速、简单地评价DNA聚合酶β的修复合成。
DNA polymerases induced by Herpes simplex and Vaccinia viruses are inhibited by aphidicolin and this inhibition is probably the basis of its antiviral activity in vivo. Its possible clinical use is however hampered by the concomitant effect on human replicative DNA polymerase α. The inhibition of human α-polymerase is reversible both in vitro and in vivo and the changes in the rate of incorporation of thymidine into DNA, following treatment with aphidicolin for a generation time, indicate the likely synchronization of the cells due to this agent. DNA polymerase β, which has recently been shown to carry out repair synthesis of damaged nuclear DNA, is not inhibited by aphidicolin either in vitro on in vivo suggesting that the drug could allow a rapid and simple evaluation of DNA repair synthesis due to DNA polymerase β.