Demonstration of decreased activity of dorsal raphe nucleus neurons in depressed suicidal patients by the AgNOR staining method
Demonstration of decreased activity of dorsal raphe nucleus neurons in depressed suicidal patients by the AgNOR staining method
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DOI：
10.1016/j.jad.2008.03.002
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发表时间：
2008-12-01
影响因子：
6.6
通讯作者：
Bogerts, Bernhard
中科院分区：
文献类型：
--
作者：
Gos, Tomasz;Krell, Dieter;Bogerts, Bernhard
背景：自杀和抑郁是密切相关但又截然不同的现象。在这两种现象中，研究都集中在石油中枢5-羟色胺能系统的紊乱上。中缝背核(DRN)是边缘结构5-羟色胺能神经支配的主要来源，对情绪影响行为的调节至关重要。方法：对23例抑郁症患者(自杀12例，非自杀11例，对照组26例)的石蜡包埋脑进行研究。结果：累加分析显示自杀对背根神经节各亚核的核面积有显著影响(方差分析，P=0.032)。自杀组这一参数的平均值低于对照组(t检验，P=0.032)。这一效应在暴力自杀受害者中尤其明显(t检验，P=0.001)，与对照组相比，他们也显示出AgNOR面积的减少(t检验。P=0.007)。未发现抑郁或极性对AgNOR参数有显著影响。这项研究的一个主要局限性是病例数量相对较少。结论：我们的研究结果表明，DRN神经元的低活动是抑郁症中的一种明显的现象，仅限于自杀的抑郁症患者亚群。(C)2008爱思唯尔B.V.保留所有权利。
Background: Suicide and depression are closely related yet distinct phenomena. In both these phenomena, research has focused oil central serotonergic system disturbances. The dorsal raphe nucleus (DRN) is the main source of serotonergic innervation of limbic structures crucial for the regulation of emotionally influenced behaviour.Methods: The study was carried Out On paraffin-embedded brains from 23 depressed patients (12 Suicides and 11 non-suicides and 26 matched controls without mental disorders. The karyometric parameters of DRN neurons were evaluated by the AgNOR silver staining method.Results: The significant effect Of Suicide on the nuclear area was found in the cumulative analysis of all DRN subnuclei (ANOVA, P=0.032). A decreased mean value of this parameter was observed in the suicides group Versus Controls (t-test, P=0.032). This effect was especially pronounced in the violent Suicide victims (t-test, P=0.001), who also demonstrated a decreased AgNOR area versus controls (t-test. P=0.007). No significant effect of depression or polarity on AgNOR parameters was found.Limitations. A major limitation of this study is relatively small case number. A further limitation is given by the lack of data on drug exposure across the whole life span.Conclusion: Our findings suggest that hypoactivity of DRN neurons is a distinct phenomenon in depression, specific only for suicidal Subgroup of depressed patients. (C) 2008 Elsevier B.V. All rights reserved.