Human embryonic stem cells have a unique epigenetic signature
Human embryonic stem cells have a unique epigenetic signature
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DOI：
10.1101/gr.5319906
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发表时间：
2006-09-01
期刊：
影响因子：
7
通讯作者：
Fan, Jian-Bing
中科院分区：
文献类型：
--
作者：
Bibikova, Marina;Chudin, Eugene;Fan, Jian-Bing
人胚胎干细胞（hES）起源于活跃的表观遗传重塑的胚胎期。DNA甲基化模式对于它们的自我更新和多功能性可能是至关重要的。我们比较了1536个CpG位点（来自371个基因）的DNA甲基化状态，在14个独立分离的hES细胞系与其他5种细胞类型：24个癌细胞系，4个成人干细胞群，4个淋巴母细胞样细胞系，5个正常人体组织，和胚胎癌细胞系。我们发现，DNA甲基化图谱清楚地将hES细胞与所有其他细胞类型区分开来。来自40个基因的49个CpG位点的子集对细胞类型之间的差异贡献最大。另一组来自23个基因的25个位点将hES细胞与正常分化的细胞区分开来，并且可以用作生物标志物来监测分化。我们的研究结果表明，hES细胞具有独特的表观遗传特征，可能有助于其发育潜力。
Human embryonic stem (hES) cells originate during an embryonic period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self- renewal and pluripotence. We compared the DNA methylation status of 1536 CpG sites (from 371 genes) in 14 independently isolated hES cell lines with five other cell types: 24 cancer cell lines, four adult stem cell populations, four lymphoblastoid cell lines, five normal human tissues, and an embryonal carcinoma cell line. We found that the DNA methylation profile clearly distinguished the hES cells from all of the other cell types. A subset of 49 CpG sites from 40 genes contributed most to the differences among cell types. Another set of 25 sites from 23 genes distinguished hES cells from normal differentiated cells and can be used as biomarkers to monitor differentiation. Our results indicate that hES cells have a unique epigenetic signature that may contribute to their developmental potential.