Staphylococcal lipases:: molecular characterisation, secretion, and processing
Staphylococcal lipases:: molecular characterisation, secretion, and processing
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DOI：
10.1016/s0009-3084(98)00025-5
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发表时间：
1998-06-01
影响因子：
3.4
通讯作者：
Rosenstein, R
中科院分区：
文献类型：
--
作者：
Götz, F;Verheij, HM;Rosenstein, R
到目前为止，已经克隆了5个不同的葡萄球菌脂肪酶基因，其中两个是金黄色葡萄球菌(Sal-L和Sal-2)，两个是表皮葡萄球菌(Sel-1和Sel-2)，一个是猪葡萄球菌(Shl)。所有相应的蛋白质都被组织成前酶：前区代表信号肽，前区的长度在207到267个氨基酸之间，成熟部分由380到400个氨基酸组成。我们发现，脂肪酶以前脂肪酶的形式分泌。通过一种特定的蛋白酶在胞外对成熟的酶进行加工。有趣的是，与成熟的脂肪酶相比，前脂肪酶显示的活性并不低。有证据表明，前区域作为分子内的伴侣，不仅促进了天然脂肪酶的易位，也促进了许多与前肽融合的完全不相关的蛋白质的易位。还观察到，亲和区保护蛋白质不被蛋白质降解。金黄色葡萄球菌和表皮葡萄球菌脂肪酶只具有脂肪酶(酯酶)活性，而相关的猪葡萄球菌酶(SHL)则同时具有脂肪酶和磷脂酶活性。这些脂肪酶的生化和催化性质在所附文章(Simons，J.W.，Gotz，F.，Egmont，M.R.和Verheij，H.M.，1998)中描述。葡萄球菌脂肪酶：生化特性。随附的文章)。(C)1998爱思唯尔爱尔兰科学有限公司。保留所有权利。
Up to date five different staphylococcal lipase genes, two of Staphylococcus aureus (sal-l and sal-2), two of Staphylococcus epidermidis (sel-1 and sel-2) and one of Staphylococcus hyicus (shl) have been cloned and sequenced. All corresponding proteins are organised as pre-pro-enzymes: the pre-region represents the signal peptide, the pro-region has a length between 207 and 267 amino acids, and the mature part comprises 380 to 400 amino acids. We found that the lipases are secreted in the pro-lipase form. The processing of the pro-form to the mature enzyme occurs extracellular by a specific protease. Interestingly the pro-lipase reveals not much less activity compared to the mature lipase. There are evidences that the pro-region acts as an intramolecular chaperone which facilitates translocation not only of the native lipase but also of a number of completely unrelated proteins fused to the pro-peptide. It was also observed that the pro-region protects the proteins from proteolytic degradation. While the Staphylococcus aureus and Staphylococcus epidermidis lipases have only lipase (esterase) activity, the related Staphylococcus hyicus enzyme (SHL) is distinguished by both lipase and phospho-lipase activity. The biochemical and catalytic properties of these lipases are described in the accompanying article (Simons, J.W., Gotz, F., Egmont, M.R. and Verheij, H.M., 1998. Staphylococcal lipases: Biochemical properties. Accompanying article). (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.