A cell-autonomous, ubiquitous marker for the analysis of Drosophila genetic mosaics.
A cell-autonomous, ubiquitous marker for the analysis of Drosophila genetic mosaics.
复制标题
用于分析果蝇遗传嵌合体的细胞自主、普遍存在的标记。
DOI：
10.1006/dbio.1994.1203
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发表时间：
1994
影响因子：
2.7
通讯作者：
O'Farrell,PH
中科院分区：
文献类型：
--
作者：
Vincent,JP;Girdham,CH;O'Farrell,PH
镶嵌分析是对含有两种不同基因型细胞的动物的研究，已被用于解决发育生物学中的大量问题。到目前为止，用于区分两种基因型细胞的细胞标记物仅适用于特定的实验情况（例如，只有成年人的翅膀）。我们设计了一个通用的细胞标记镶嵌分析。它由在果蝇犰狳基因的组成型启动子控制下表达的细菌LacZ基因组成。携带该融合基因的转化体在所有组织中表达β-半乳糖苷酶，并在分析的所有阶段表达。合子表达早在原肠胚形成时就可检测到。在通过核移植获得的嵌合体中，携带转基因的细胞容易与β-半乳糖苷酶阴性宿主细胞区分开。该标记物还应该对用“Flp技术”产生的镶嵌物有用。"
Mosaic analysis, the study of animals containing cells of two different genotypes, has been used to address a wealth of questions in developmental biology. Up to now, the cell markers used to distinguish cells of the two genotypes have only been applicable to specific experimental situations (e.g., only in adult wings). We have designed a general purpose cell marker for mosaic analysis. It consists of the bacterialLacZgene expressed under the control of the constitutive promoter of the Drosophilaarmadillogene. Transformants carrying this fusion gene express β-galatocsidase in all tissue and at all stages analyzed. Zygotic expression is detectable as early as gastrulation. In mosaics obtained by nuclear transplantation, cells carrying the transgene are easily distinguished from β-galactosidase-negative host cells. The marker should also be useful for mosaics generated with the "Flp technique."