Loss of alkaline ceramidase inhibits autophagy in Arabidopsis and plays an important role during environmental stress response
Loss of alkaline ceramidase inhibits autophagy in Arabidopsis and plays an important role during environmental stress response
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碱性神经酰胺酶的缺失会抑制拟南芥的自噬，并在环境应激反应中发挥重要作用
DOI：
10.1111/pce.13148
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yao Nan（姚楠）
中科院分区：
文献类型：
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作者：
Zheng Ping;Wu Jian-Xin;Sahu Sunil Kumar;Zeng Hong-Yun;Huang Li-Qun;Liu Zhe;Xiao Shi;Yao Nan（姚楠）
鞘脂是细胞膜中发现的一类生物活性脂质，可以调节细胞膜的生物物理特性，并在信号转导中发挥关键作用。鞘脂参与人类和酵母的自噬，但其在植物自噬中的作用尚不清楚。在这项研究中，我们报道了AtACER，一种将神经酰胺水解为长链碱（LCB）的碱性神经酰胺酶，在拟南芥的自噬过程中发挥作用。我们的经验数据表明，AtACER 的缺失会抑制自噬，而其过度表达会在营养、盐度和氧化应激下促进自噬。有趣的是，缺氮显着影响拟南芥中鞘脂的分布，尤其是 LCB。此外，外源应用 LCB 也会诱导自噬。我们的研究结果揭示了AtACER的一个新功能，它被发现参与自噬过程，因此，在维持鞘脂和自噬之间的动态循环以维持各种环境压力下的细胞稳态方面发挥着至关重要的作用。
Sphingolipids, a class of bioactive lipids found in cell membranes, can modulate the biophysical properties of the membranes and play a critical role in signal transduction. Sphingolipids are involved in autophagy in humans and yeast, but their role in autophagy in plants is not well understood. In this study, we reported that theAtACER, an alkaline ceramidase that hydrolyses ceramide to long‐chain base (LCB), functions in autophagy process in Arabidopsis. Our empirical data showed that the loss ofAtACERinhibited autophagy, and its overexpression promoted autophagy under nutrient, salinity, and oxidative stresses. Interestingly, nitrogen deprivation significantly affected the sphingolipid's profile inArabidopsis thaliana, especially the LCBs. Furthermore, the exogenous application of LCBs also induced autophagy. Our findings revealed a novel function ofAtACER, where it was found to involve in the autophagy process, thus, playing a crucial role in the maintenance of a dynamic loop between sphingolipids and autophagy for cellular homeostasis under various environmental stresses.