Insight into the interaction of human serum albumin with folic acid: A biophysical study
Insight into the interaction of human serum albumin with folic acid: A biophysical study
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DOI：
10.1016/j.saa.2018.06.093
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发表时间：
2018-11-05
影响因子：
4.4
通讯作者：
Florescu, Monica
中科院分区：
文献类型：
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作者：
Chilom, Claudia G.;Bacalum, Mihaela;Florescu, Monica
叶酸（Folic acid，FA）是维生素B族复合物（B 9）中的一种水溶性生物活性食品成分。FA缺乏可导致多种人类健康问题，而高摄入FA可降低自然杀伤细胞的细胞毒性。本研究的主要目的是探讨在生理pH值下，使用ATR-FTIR，荧光光谱，循环伏安法和电化学阻抗谱的FA与人血清白蛋白（HSA）的相互作用，以了解HSA作为FA在水溶液中的血液转运体的作用，可用于不同的治疗。随后在FA存在下对HSA进行淬灭，并测定结合常数（K-b）。电化学参数的变化证明了FA与固定化HSA的结合，并且当通过荧光猝灭研究时，结合常数是溶液中自由分子之间发生相互作用时所获得的值的十倍。这些结果可以用于未来的研究，以改善药物输送系统或叶酸和食物成分结合到HSA纳米颗粒或纳米胶囊的细胞摄取。(C)2018 Elsevier B. V.版权所有。
Folic acid (FA) is a water soluble bioactive food constituent from the vitamin B-family complex (B9). FA deficiency can lead to a variety of human health problems, while a high intake of FA can reduce the cytotoxicity of natural killer cells. The main goal of this study was to investigate the interaction of FA with human serum albumin (HSA) at physiological pH using ATR-FTIR, fluorescence spectroscopy, cyclic voltammetry and electrochemical impedance spectroscopy in order to understand the role of HSA as a blood transporter for FA in aqueous solution that can be used in different therapies. The quenching of HSA in the presence of FA was followed and the binding constant (K-b) was determined. The variation of electrochemical parameters proved that the FA binds to immobilized HSA and the binding constant was ten times than the value obtained when the interaction takes place between free molecules in solution when studied by fluorescence quenching. The results can be used in future studies to improve drug delivery systems or cellular uptake of folic add and food components conjugated to HSA nanoparticles or nanocapsules. (C) 2018 Elsevier B.V. All rights reserved.