Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398.
Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398.
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通过 miR398 环境温度增强菊花 CdICE1 拟南芥的冷冻耐受性
DOI：
10.1186/1741-7007-11-121
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发表时间：
2013-12-19
期刊：
影响因子：
5.4
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Chen Y;Jiang J;Song A;Chen S;Shan H;Luo H;Gu C;Sun J;Zhu L;Fang W;Chen F
背景ICE(Inducer Of CBF Expression)家族基因在耐冷途径的调控中发挥着重要作用。在早期的研究中，我们从菊花中分离到了CDICE1基因，并证明了过表达CDICE1可以提高植物的耐寒性。结果通过EMSA和酵母单杂交实验，我们证实了CDICE1能与CDDREBA启动子中的MYC元件特异结合并激活转录。此外，在从23℃过渡到4℃或16℃后，过表达的cdICE1提高了拟南芥的耐寒性。我们发现，在4℃驯化后，cdICE1和拟南芥AtICE1一样，促进了CBF(CRT/DRE结合因子)及其下游与耐寒性相关的基因的表达，包括COR15a(冷调节15a)、COR6.6和RD29a(对干旱29a的响应)。有趣的是，我们观察到在miR398中过表达CDICE1的植物显著减少。此外，其靶基因CSD1(铜/锌超氧化物歧化酶1)和CSD2在16℃驯化条件下可诱导表达，表明miR398-CSD途径参与了抗冻性的诱导。结论在两种不同的温度驯化条件下，CDICE1介导的抗冻性通过不同的途径发生，涉及CBF或miR398。
BackgroundICE (Inducer of CBF Expression) family genes play an important role in the regulation of cold tolerance pathways. In an earlier study, we isolated the gene CdICE1 from Chrysanthemum dichrum and demonstrated that freezing tolerance was enhanced by CdICE1 overexpression. Therefore, we sought to determine the mechanism by which ICE1 family genes participate in freezing tolerance.ResultsUsing EMSA (Electrophoretic Mobility Shift Assay) and yeast one-hybrid assays, we confirmed that CdICE1 binds specifically to the MYC element in the CdDREBa promoter and activates transcription. In addition, overexpression of CdICE1 enhanced Arabidopsis freezing tolerance after transition from 23°C to 4°C or 16°C. We found that after acclimation to 4°C, CdICE1, like Arabidopsis AtICE1, promoted expression of CBFs (CRT/DRE Binding Factor) and their genes downstream involved in freezing tolerance, including COR15a (Cold-Regulated 15a), COR6.6, and RD29a (Responsive to Dessication 29a). Interestingly, we observed that CdICE1-overexpressing plants experienced significant reduction in miR398. In addition, its target genes CSD1 (Copper/zinc Superoxide Dismutase 1) and CSD2 showed inducible expression under acclimation at 16°C, indicating that the miR398-CSD pathway was involved in the induction of freezing tolerance.ConclusionsOur data indicate that CdICE1-mediated freezing tolerance occurs via different pathways, involving either CBF or miR398, under acclimation at two different temperatures.