Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions
Influence of the photoperiod on redox regulation and stress responses in Arabidopsis thaliana L. (Heynh.) plants under long- and short-day conditions
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DOI：
10.1007/s00425-006-0222-3
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发表时间：
2006-07-01
期刊：
影响因子：
4.3
通讯作者：
Scheibe, Renate
中科院分区：
文献类型：
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作者：
Becker, Beril;Holtgrefe, Simone;Scheibe, Renate
拟南芥（Heynh.）植物在短日照（7.5小时光周期）或长日照（16小时光周期）下在低光照（150 μ mol光子m（-2）s（-1）和20 ℃）下生长，然后转入高光照和低温（350-800 μ mol光子m（-2）s（-1），12 ℃）下。在短时间内生长的植物响应NADP-苹果酸脱氢酶（EC 1.1.1.82）活化状态的快速增加。然而，持续的过度复位揭示了苹果酸瓣调节的新水平。活性测量和Northern-blot分析表明，NADP-苹果酸脱氢酶的转录和蛋白质水平在几个小时内增加。使用宏阵列，基因表达的其他变化进行了鉴定。谷胱甘肽代谢的几种酶和一些光合作用基因的转录水平增加。细胞谷胱甘肽水平增加，但其氧化还原状态保持不变。在长日照条件下生长的植物中观察到不同的情况。NADP-苹果酸脱氢酶和谷胱甘肽含量都没有变化，但几种抗氧化酶的表达强烈增加。我们的结论是，内源性系统，测量日长与氧化还原调节相互作用，并推翻了信号的解释，即它们重定向氧化还原介导的驯化信号，以允许更有效的光的使用和氧化还原中毒在短天的系统，以防止氧化损伤时，在长日条件下生长。
Arabidopsis thaliana L. (Heynh.) plants were grown in low light (150 mu mol photons m(-2) s(-1) and 20 degrees C) either in short days (7.5 h photoperiod) or long days ( 16 h photoperiod), and then transferred into high light and low temperature (350-800 mu mol photons m(-2) s(-1) at 12 degrees C). Plants grown in short days responded with a rapid increase in NADP-malate dehydrogenase (EC 1.1.1.82) activation state. However, persisting overreduction revealed a new level of regulation of the malate valve. Activity measurements and Northern-blot analyses indicated that NADP-malate dehydrogenase transcript and protein levels increased within a few hours. Using macroarrays, additional changes in gene expression were identified. Transcript levels for several enzymes of glutathione metabolism and of some photosynthetic genes increased. The cellular glutathione level increased, but its redox state remained unchanged. A different situation was observed in plants grown in long-day conditions. Neither NADP-malate dehydrogenase nor glutathione content changed, but the expression of several antioxidative enzymes increased strongly. We conclude that the endogenous systems that measure day length interact with redox regulation, and override the interpretation of the signals, i.e. they redirect redox-mediated acclimation signals to allow for more efficient light usage and redox poising in short days to systems for the prevention of oxidative damages when grown under long-day conditions.