E-spun composite fibers of collagen and dragline silk protein: fiber mechanics, biocompatibility, and application in stem cell differentiation.
E-spun composite fibers of collagen and dragline silk protein: fiber mechanics, biocompatibility, and application in stem cell differentiation.
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DOI：
10.1021/bm501403f
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发表时间：
2015-01-12
影响因子：
6.2
通讯作者：
Wang, Rong
中科院分区：
文献类型：
--
作者：
Zhu, Bofan;Li, Wen;Lewis, Randolph V.;Segre, Carlo U.;Wang, Rong
生物复合材料具有高机械强度、高稳定性和定向基质干细胞分化的能力，是组织工程和细胞治疗中重建受损组织所必需的。为此，我们使用静电纺丝技术，从转基因山羊的乳汁中获得胶原蛋白和蜘蛛牵引丝蛋白，在类似规模的情况下模拟天然细胞外基质(ECM)，制备出排列良好的复合纤维。研究发现，在电纺(E-Spun)纤维中，胶原蛋白和牵引丝蛋白以各种比例均匀混合。结果表明，随着含丝率的增加，纤维的极限拉伸强度和弹性单调增加，伸长率略有下降。值得注意的是，我们发现，丝蛋白与胶原蛋白的结合显著提高了基质的稳定性，使其不受细胞培养液中纤维过度膨胀和形状变形的影响。将人顶蜕膜胎盘干细胞(HdpPSCs)种植在胶原-丝素基质上，发现该基质支持细胞增殖的速度与纯胶原基质相似，但它们提供的细胞黏附强度降低，并在不同程度上诱导细胞极化。含有15wt%和30wt%丝素的胶原基质(CS15，CS30)诱导的神经分化水平与纯胶原相当。尤其是CS15基质诱导的细胞极化程度最大，并促进了延长的一维神经丝的发育，与排列的纤维严格平行。考虑到增强的机械强度和纤维的稳定性，CS15和CS30 E纺纤维提供了更好的替代纯胶原纤维的支架，可用于神经组织修复和未来纳米生物装置的开发。
Biocomposite matrices with high mechanical strength, high stability, and the ability to direct matrix-specific stem cell differentiation are essential for the reconstruction of lesioned tissues in tissue engineering and cell therapeutics. Toward this end, we used the electrospinning technique to fabricate well-aligned composite fibers from collagen and spider dragline silk protein, obtained from the milk of transgenic goats, mimicking the native extracellular matrix (ECM) on a similar scale. Collagen and the dragline silk proteins were found to mix homogeneously at all ratios in the electrospun (E-spun) fibers. As a result, the ultimate tensile strength and elasticity of the fibers increased monotonically with silk percentage, whereas the stretchability was slightly reduced. Strikingly, we found that the incorporation of silk proteins to collagen dramatically increased the matrix stability against excessive fiber swelling and shape deformation in cell culture medium. When human decidua parietalis placental stem cells (hdpPSCs) were seeded on the collagen–silk matrices, the matrices were found to support cell proliferation at a similar rate as that of the pure collagen matrix, but they provided cell adhesion with reduced strengths and induced cell polarization at varied levels. Matrices containing 15 and 30 wt % silk in collagen (CS15, CS30) were found to induce a level of neural differentiation comparable to that of pure collagen. In particular, CS15 matrix induced the highest extent of cell polarization and promoted the development of extended 1D neural filaments strictly in-line with the aligned fibers. Taking the increased mechanical strength and fiber stability into consideration, CS15 and CS30 E-spun fibers offer better alternatives to pure collagen fibers as scaffolds that can be potentially utilized in neural tissue repair and the development of future nanobiodevices.
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影响因子：
4.6
作者：
Hu, Xiao;Raja, Waseem K.;An, Bo;Tokareva, Olena;Cebe, Peggy;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
1.8
作者：
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作者：
CHEUNG, DT;PERELMAN, N;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
6.2
作者：
Bini, Elisabetta;Foo, Cheryl Wong Po;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
64.5
作者：
Emsley, J;Knight, CG;Liddington, RC
通讯作者：
Liddington, RC