Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves
Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves
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DOI：
10.1111/j.1574-6968.1999.tb08794.x
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发表时间：
1999-11-15
影响因子：
2.1
通讯作者：
Dehorter, B
中科院分区：
文献类型：
--
作者：
Berto, P;Comménil, P;Dehorter, B
根据我们的研究结果，芸苔链格孢菌是一种病原体，由于几种丝氨酸酯酶，它可以直接穿透寄主角质层。其中，通过SDS-PAGE和免疫印迹法在未萌发孢子的水洗液中检测到80-kDa脂肪酶（E. C3.1.1.3）。纯化的脂肪酶与灰葡萄孢抗脂肪酶抗体发生交叉反应，据报道，这些抗体在体外抑制脂肪酶活性。将抗脂肪酶抗体加入到A.在接种之前接种Brassicicola。因此，黑点病变减少了90%，完整的花椰菜叶片，但不是从表面蜡已被删除的叶子。孢子表面结合的脂肪酶被认为与表皮叶蜡密切相互作用，用于在宿主-寄生虫相互作用的早期阶段粘附和/或穿透真菌繁殖体。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1.3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted with Botrytis cinerea anti-lipase antibodies, which were reported to inhibit the in vitro lipase activity. Anti-lipase antibodies were added to a conidial suspension of A. brassicicola prior to inoculation. As a result, blackspot lesions were reduced by 90% on intact cauliflower leaves, but not on leaves from which surface wax had been removed. Spore surface bound lipase is thought to interact closely with epicuticular leaf waxes for adhesion and/or penetration of the fungal propagules during the early stages of host-parasite interactions. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.