Site-specific cleavage of DNA-RNA hybrids by zinc finger FokI cleavage domain fusions
Site-specific cleavage of DNA-RNA hybrids by zinc finger FokI cleavage domain fusions
复制标题
DOI：
10.1016/s0378-1119(97)00489-7
复制
发表时间：
1997-12-05
期刊：
影响因子：
3.5
通讯作者：
Chandrasegaran, S
中科院分区：
文献类型：
--
作者：
Kim, YG;Shi, YG;Chandrasegaran, S
Cys(2)His(2)型锌指蛋白与DNA - RNA杂合体的结合亲和力与它们和DNA双链体的结合亲和力相当。通过将这类锌指蛋白与FokI切割结构域融合，它们被转化为位点特异性切割酶。融合蛋白具有活性，在最佳条件下能以序列特异性方式切割DNA双链体。这些融合蛋白也表现出对DNA - RNA杂合体内DNA链的位点特异性切割，尽管与切割DNA双链体相比效率较低（约为或等于50倍）。就其DNA - RNA切割特性而言，这些经过改造的内切核酸酶是此类酶中的首创，它们可能具有重要的生物学应用。（C）1997年，爱思唯尔科学出版社（Elsevier Science B.V.）
Zinc-finger proteins of the Cys(2)His(2) type bind DNA-RNA hybrids with affinities comparable to those for DNA duplexes. Such zinc-finger proteins were converted into site-specific cleaving enzymes by fusing them to the FokI cleavage domain. The fusion proteins are active and under optimal conditions cleave DNA duplexes in a sequence-specific manner. These fusions also exhibit site-specific cleavage of the DNA strand within DNA-RNA hybrids albeit at a lower efficiency (similar or equal to 50-fold) compared to the cleavage of the DNA duplexes. These engineered endonucleases represent the first of their kind in terms of their DNA-RNA cleavage properties, and they may have important biological applications. (C) 1997 Elsevier Science B.V.