Peroxisome proliferator-activated receptor delta expression and regulation in mouse uterus during embryo implantation and decidualization
Peroxisome proliferator-activated receptor delta expression and regulation in mouse uterus during embryo implantation and decidualization
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DOI：
10.1002/mrd.10348
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发表时间：
2003-11-01
影响因子：
2.5
通讯作者：
Yang, ZM
中科院分区：
文献类型：
--
作者：
Ding, NZ;Teng, CB;Yang, ZM
采用原位杂交和免疫组织化学方法研究小鼠妊娠早期子宫组织中过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor，PPARdelta）基因的表达及其调控。还研究了在假子宫内膜异位症、延迟着床、激素治疗和人工蜕膜化下的PPARdelta表达。在妊娠第1-4天有非常低水平的PPARdelta表达。胚胎植入第5天，仅在植入囊胚周围的腔下基质中观察到PPARdelta表达。妊娠第5天子宫内未见相应信号。在延迟植入下没有可检测到的PPARdelta信号。一旦通过雌激素处理终止延迟植入并植入胚胎，在植入胚泡周围的腔下基质中诱导强水平的PPARdelta表达。雌激素治疗诱导了中度水平的PPARdelta表达的腺上皮细胞，而孕酮治疗卵巢切除小鼠没有影响。在妊娠第6-8天的蜕膜中观察到强水平的PPARdelta表达。在人工蜕膜化下也诱导PPARdelta表达。这些数据表明，PPARdelta在植入部位的表达需要一个活跃的囊胚的存在，并可能发挥重要作用的囊胚植入。(C)2003 Wiley-Liss，Inc.
The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and artificial decidualization was also investigated. There was a very low level of PPARdelta expression on days 1-4 of pregnancy. On day 5 when embryo implanted, PPARdelta expression was exclusively observed in the subluminal stroma surrounding the implanting blastocyst. No corresponding signals were seen in the uterus on day 5 of pregnancy. There was no detectable PPARdelta signal under delayed implantation. Once delayed implantation was terminated by estrogen treatment and embryo implanted, a strong level of PPARdelta expression was induced in the subluminal stroma surrounding the implanting blastocyst. Estrogen treatment induced a moderate level of PPARdelta expression in the glandular epithelium, while progesterone treatment had no effects in the ovariectomized mice. A strong level of PPARdelta expression was seen in the decidua on days 6-8 of pregnancy. PPARdelta expression was also induced under artificial decidualization. These data suggest that PPARdelta expression at implantation sites require the presence of an active blastocyst and may play an essential role for blastocyst implantation. (C) 2003 Wiley-Liss, Inc.