Visible light-induced destabilization of endocytosed liposomes
Visible light-induced destabilization of endocytosed liposomes
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DOI：
10.1016/s0014-5793(00)01122-4
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发表时间：
2000-02-04
期刊：
影响因子：
3.5
通讯作者：
O'Brien, DF
中科院分区：
文献类型：
--
作者：
Miller, CR;Clapp, PJ;O'Brien, DF
脂质体的光诱导去稳定化的潜在生物医学用途部分地取决于使用具有其固有治疗优点的绿色至近红外光。双层的聚合可以通过将选择的两亲性花青染料，即阳离子1，1 ′-双十八烷基-3，3，3 ′，3 ′-四甲基吲哚羰花青（DiI），或相应的阴离子二磺化DiI（DiI-DS）与脂质双层。1，2-二油酰基-sn-甘油基-3-磷酸乙醇胺/1，2-双[10-（2 '，4'-己二烯酰氧基）-癸酰基]-sn-甘油基-3-磷酸胆碱脂质体聚合的DiI敏化作用导致脂质体不稳定，释放包封的水性标记物。在单独的实验中，类似的光敏脂质体被培养的HeLa细胞内吞。将细胞和脂质体暴露于550 nm光引起脂质体包封的8-羟基芘-1，3，6-三磺酸（HPTS）从低pH隔室净移动到较高pH隔室。这表明DiI标记的脂质体的光解导致内吞脂质体的内容物递送至细胞质。HPTS释放到细胞质中似乎需要标记的脂质体与内体膜的光活化融合，这些研究有助于设计可见光敏感的脂质体，用于将脂质体包裹的试剂递送到细胞质中。(C)2000年欧洲生物化学学会联合会。
The potential biomedical utility of the photoinduced destabilization of liposomes depends in part on the use of green to near infrared light with its inherent therapeutic advantages, The polymerization of bilayers can be sensitized to green light by associating selected amphiphilic cyanine dyes, i.e. the cationic 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI), or the corresponding anionic disulfonated DiI (DiI-DS), with the lipid bilayer. The DiI sensitization of the polymerization of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine/1,2-bis[10-(2',4'-hexadienoyloxy)-decanoyl]-sn-glycero-3-phosphocholine liposomes caused liposome destabilization with release of encapsulated aqueous markers. In separate experiments, similar photosensitive liposomes were endocytosed by cultured HeLa cells, Exposure of the cells and liposomes to 550 nm light caused a net movement of the liposome-encapsulated 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) from low pH compartment(s) to higher pH compartment(s). This suggests that photolysis of DiI-labelled liposomes results in delivery of the contents of the endocytosed liposomes to the cytoplasm. The release of HPTS into the cytoplasm appears to require the photoactivated fusion of the labelled liposomes with the endosomal membrane, These studies aid in the design of visible light sensitive liposomes for the delivery of liposome-encapsulated reagents to the cytoplasm. (C) 2000 Federation of European Biochemical Societies.