Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative.
Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative.
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DOI：
10.1038/s41598-017-14725-x
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发表时间：
2017-10-30
影响因子：
4.6
通讯作者：
Pastorin G
中科院分区：
文献类型：
--
作者：
Goh WJ;Zou S;Ong WY;Torta F;Alexandra AF;Schiffelers RM;Storm G;Wang JW;Czarny B;Pastorin G
细胞衍生纳米囊泡(CDN)是从迅速扩展的外体领域发展而来的，代表了一类生物启发的药物递送系统(DDS)。然而，在分离自然分泌的外切体方面，由于产量低且分步骤进行，限制了将其转化为临床应用。在这里，我们首次展示了一种使用旋转杯通过细胞剪切法简单而快速地生产CDN的方法，该方法在产量和成本效益方面都明显优于传统的外体分离和现有的CDN制造技术。得到的CDN具有更高的蛋白质产量，在物理性质、蛋白质和脂肪分析方面与文献报道的Exosome和CDN相似。此外，我们还研究了CDN在体外的细胞摄取机制及其在小鼠体内肿瘤模型中的生物分布。在肿瘤小鼠模型中，CDN在肿瘤部位共定位，突出了CDN作为抗癌策略的潜力。综上所述，这些结果表明，CDNS可以作为理想的药物纳米载体提供一种成本效益高的替代外切体的方法。
Cell Derived Nanovesicles (CDNs) have been developed from the rapidly expanding field of exosomes, representing a class of bioinspired Drug Delivery Systems (DDS). However, translation to clinical applications is limited by the low yield and multi-step approach in isolating naturally secreted exosomes. Here, we show the first demonstration of a simple and rapid production method of CDNs using spin cups via a cell shearing approach, which offers clear advantages in terms of yield and cost-effectiveness over both traditional exosomes isolation, and also existing CDNs fabrication techniques. The CDNs obtained were of a higher protein yield and showed similarities in terms of physical characterization, protein and lipid analysis to both exosomes and CDNs previously reported in the literature. In addition, we investigated the mechanisms of cellular uptake of CDNs in vitro and their biodistribution in an in vivo mouse tumour model. Colocalization of the CDNs at the tumour site in a cancer mouse model was demonstrated, highlighting the potential for CDNs as anti-cancer strategy. Taken together, the results suggest that CDNs could provide a cost-effective alternative to exosomes as an ideal drug nanocarrier.
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外泌体作为帕金森氏病疗法的药物。
DOI：
10.1016/j.jconrel.2015.03.033
发表时间：
2015-06-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Haney MJ;Klyachko NL;Zhao Y;Gupta R;Plotnikova EG;He Z;Patel T;Piroyan A;Sokolsky M;Kabanov AV;Batrakova EV
通讯作者：
Batrakova EV
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DOI：
10.3390/ph6050659
发表时间：
2013
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Marcus ME;Leonard JN
通讯作者：
Leonard JN
影响因子：
8
作者：
Goh, Wei Jiang;Lee, Choon Keong;Pastorin, Giorgia
通讯作者：
Pastorin, Giorgia
DOI：
10.1016/j.bbrc.2015.03.172
发表时间：
2015-05-22
影响因子：
3.1
作者：
Ban, Jae-Jun;Lee, Mijung;Kim, Manho
通讯作者：
Kim, Manho
影响因子：
8
作者：
Kooijmans SA;Vader P;van Dommelen SM;van Solinge WW;Schiffelers RM
通讯作者：
Schiffelers RM