Distribution, shape, and immnohistochemical characteristics of serotonin-immunoreactive neuroendocrine cells in the urethra and periurethral genital organs in mice
Distribution, shape, and immnohistochemical characteristics of serotonin-immunoreactive neuroendocrine cells in the urethra and periurethral genital organs in mice
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小鼠尿道和尿道周围生殖器官中血清素免疫反应性神经内分泌细胞的分布、形态和免疫组织化学特征
DOI：
10.1007/s10735-021-10020-2
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发表时间：
2021
影响因子：
3.2
通讯作者：
Kei-ichiro Nakamura
中科院分区：
文献类型：
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作者：
Kei-ichiro Uemura;Tasuku Hiroshge;Kosuke Ueda;Keisuke Ohta;Tokumasa Hayashi;Shingo Hirashima;Tomonoshin Kanazawa;Makoto Nakiri;Tsukasa Igawa;Kei-ichiro Nakamura
本研究的目的是通过对小鼠尿道连续光镜切片的组织学和免疫组织化学分析，阐明小鼠前列腺及其周围生殖器官中降钙素免疫反应神经内分泌细胞（SIR-NECs）的分布、形态和免疫组织化学特征。取13周龄小鼠下尿路标本，光镜下观察SIR-NECs的形态和分布。将切片上的器官分为三个解剖区以阐明SIR-NEC的分布模式：（1）A区，前列腺尿道附近的导管;（2）B区，尿道括约肌外的导管;和（3）C区，腺泡区域。切片用抗5-羟色胺抗体和神经内分泌相关因子（NRF）抗体进行双重免疫染色，NRF包括10种神经细胞标志物和8种神经递质，以及4′，6-二氨基-2-苯基吲哚（DAPI）。此外，SIR-NEC用抗细胞角蛋白5（CK 5）和p63的抗体以及DAPI进行双重免疫染色。SIR-NECs主要分布在A区，C区未见SIR-NECs。在A区和B区，烧瓶形SIR-NEC的比例约为15%。尿道上皮中未见瓶状SIR-NEC。与SIR-NEC共定位的NRF有降钙素基因相关肽、CD 56、嗜铬粒蛋白A、神经元特异性烯醇化酶、神经元细胞质蛋白9.5和突触素（分别为72.3%、73.2%、88.9%、92.3%、91.7%和81.9%）。CK 5和p63不与SIR-NEC共定位。在本研究中，尿道和周围生殖器官的SIR-NEC普遍存在于尿道和尿道附近的导管中，并共表达特异性神经相关的NRFs。
The aim of this study is to clarify the disibution, shape, and immunohistochemical characteristics of serotonin-immunoreactive neuroendocrine cells (SIR-NECs) in mouse prostate and in the surrounding genital organs by histological and immunohistochemical analysis of the light microscopic serial sections of urethra. We collected lower urinary tracts from 13-week-old mice and observed the distribution pattern and shape of the SIR-NECs by serial light microscopy. The organs on the sections were divided into three anatomical zones to clarify the distribution pattern of SIR-NECs: (1) zone A, the ducts near the prostatic urethra; (2) zone B, the ducts outside the urethral sphincter; and (3) zone C, the acinus areas. Sections were double immune-stained with antibodies against serotonin and one of neuroendocrine-related factors (NRFs), including 10 neural cell markers and eight neurotransmitters, and also 4′,6-diamino-2-phenylindole (DAPI). In addition, SIR-NECs were double immune-stained with antibodies against cytokeratin 5 (CK5) and p63, together with DAPI. SIR-NECs were mostly localized in zone A, and no SIR-NECs were observed in zone C. The proportion of flask-shaped SIR-NECs was approximately 15% in zones A and B. No flask-shaped SIR-NECs were observed in urethral epithelia. The NRFs co-localized with SIR-NEC were calcitonin gene-related peptide, CD56, chromogranin A, neuron-specific enolase, neuron cytoplastic protein 9.5, and synaptophysin (72.3%, 73.2%, 88.9%, 92.3%, 91.7%, and 81.9%, respectively). CK5 and p63 were not co-localized with SIR-NECs. In this study, SIR-NEC of the urethra and the surrounding genital organs was ubiquitous in the urethra and the ducts near the urethra and co-expressed specific nerve-related NRFs.