Synthesis, Mechanical, In Vitro and In Vivo Bioactivity and Preliminary Biocompatibility Studies of Bioglasses
Synthesis, Mechanical, In Vitro and In Vivo Bioactivity and Preliminary Biocompatibility Studies of Bioglasses
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DOI：
10.1166/sam.2019.3553
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发表时间：
2019-10-01
影响因子：
0.9
通讯作者：
Yousef, El Sayed
中科院分区：
文献类型：
--
作者：
Algarni, H.;AlShahrani, Ibrahim;Yousef, El Sayed
在40P(2)O(5)20Na(2)O- 10Ca(OH)(2)- 20cacl (2)-8.0 zno -2.0MgO （BGMg）生物玻璃中对MgO氧化物纳米β -磷酸三钙支架（β - tcp）进行了评价，以改善其力学、结构和生物学性能。x射线衍射分析（XRD）表明，β - tcp的主要相和少数相被研究。测定了制备的生物玻璃的维氏显微硬度H-v。本文研究了体外对正常和活化脾细胞的抑菌活性、抗增殖/细胞毒性以及活化脾细胞对BGMg的体外细胞毒性作用和体外效应。结果表明，纳米（β - tcp）与MgO颗粒均对革兰氏阴性和阳性细菌以及真菌具有抗菌活性。结果表明，纳米颗粒（β - tcp）的抑菌效果优于粉末（β - tcp）。此外，β - tcp纳米颗粒对正常脾细胞具有细胞毒性作用，制备的生物玻璃BGMg对红细胞没有任何急性细胞毒性或溶解作用。因此，在现有的玻璃基体P2O5-Na2O-Ca(OH)(2)-CaCl2-ZnO中添加MgO可以优化和提高玻璃的生物和力学性能。
Nano beta-tricalcium phosphate scaffolds (beta-TCP) with MgO oxide were estimated in the bioglasses 40P(2)O(5)20Na(2)O- 10Ca(OH)(2)-20CaCl(2)-8.0ZnO-2.0MgO (BGMg) to improve that the mechanical, structural and biological properties. X-ray diffraction analysis (XRD) indicated that the major phase of beta-TCP and few phases of beta-TCP were investigated. The Vicker's microhardness, H-v, of prepared bioglasses was evaluated. Herein the antimicrobial activity, anti-proliferative/cytotoxicity against normal and activated splenic cells in vitro, and in vitro cytotoxicity effects and in vitro effects of activated splenic cells on BGMg. The results demonstrated that both nano (beta-TCP) with MgO particle attributed to antimicrobial activities against Gram negative and positive bacteria as well as fungi. It was obtained that the nanoparticles of (beta-TCP) were the stronger effect on the antimicrobial activity than the powder (beta-TCP). In addition (beta-TCP nanoparticles) demonstrated the cytotoxic effect on normal splenic cells and the prepared bioglasses BGMg did not cause any acute cytotoxicity or lysis to RBCs. Hence, addition MgO to the present glasses matrix P2O5-Na2O-Ca(OH)(2)-CaCl2-ZnO leads to optimize and enhancing biological and mechanical properties.