H2A.ZI, a new variant histone expressed during Xenopus early development exhibits several distinct features from the core histone H2A
H2A.ZI, a new variant histone expressed during Xenopus early development exhibits several distinct features from the core histone H2A
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DOI：
10.1093/nar/24.20.3947
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发表时间：
1996-10-15
影响因子：
14.9
通讯作者：
Mechali, M
中科院分区：
文献类型：
--
作者：
Iouzalen, N;Moreau, J;Mechali, M
我们从非洲爪蟾的消减cDNA文库中分离到一个新的转录本H2 A，ZI，它属于H2 A，Z变异基因家族，其在卵子发生和发育过程中的表达与核心组蛋白H2 A的表达有显著差异。首先，H2 A，ZI mRNA主要在卵子发生和中囊胚转换后表达，而H2 A是组成型表达，第二，与H2 A相反，变体H2A.ZI在发育过程中被聚腺苷酸化。第三，H2A.ZI的表达在原肠胚后从S期解偶联，而核心组蛋白H2 AmRNA的合成被严格控制于DNA复制。有趣的是，H2 A，ZI在N-末端尾部带较少电荷，这对于染色质介导的阻遏是至关重要的。H2 A，ZI的特征表明其掺入核小体将导致在发育期间更有能力的染色质结构。
We have isolated from a subtractive cDNA library of Xenopus laevis a novel transcript, H2A,ZI, which belongs to the H2A,Z variant gene family, Characterization of its expression during oogenesis and development shows significant differences from the expression of the core histone H2A, First, H2A,ZI mRNA is mainly detected only during oogenesis and after the midblastula transition, whereas H2A is constitutively expressed, at much higher levels, throughout embryonic growth, Second, in contrast with H2A, the variant H2A.ZI is polyadenylated during development, Third, expression of H2A,ZI is uncoupled from the S phase after gastrula, whereas synthesis of the core histone H2A mRNA is tightly controlled to DNA replication, Interestingly, H2A,ZI is less charged in the N-terminal tail which is crucial for chromatin-mediated repression, The characteristics of H2A,ZI suggest that its incorporation into nucleosomes would lead to a chromatin structure more competent fbr gene expression during development.