Unidirectional effect of lauryl sulfate on the reversible NADH:ubiquinone oxidoreductase (Complex I).
Unidirectional effect of lauryl sulfate on the reversible NADH:ubiquinone oxidoreductase (Complex I).
复制标题
月桂基硫酸盐对可逆 NADH：泛醌氧化还原酶（复合物 I）的单向作用。
DOI：
10.1016/s0014-5793(03)00765-8
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
Vinogradov,AD
中科院分区：
文献类型：
--
作者：
Grivennikova,VG;Ushakova,AV;Cecchini,G;Vinogradov,AD
十二烷基硫酸酯抑制由NADH：泛醌氧化还原酶(复合体I)催化的ΔH+依赖的反向电子转移反应，该酶位于偶联的牛心亚线粒体颗粒和脱氮副球藻来源的囊泡中。在选择性地阻止琥珀酸支持的、鱼藤酮敏感的NAD+或铁氰化物还原的浓度下，该抑制剂既不影响NADH氧化酶(偶联的或非偶联的)也不影响NADH：铁氰化物还原酶和琥珀酸氧化酶的活性。排除了抑制剂可能的解偶联作用：与寡霉素和革兰西丁在偶联和解偶联作用的同时分别增加和降低反向电子转移速率相反，十二烷基硫酸酯不影响呼吸控制率。提出了十二烷基硫酸酯对络合物I催化氧化还原的单向效应的机理模型。
Lauryl sulfate inhibits the ΔH+‐dependent reverse electron transfer reactions catalyzed by NADH:ubiquinone oxidoreductase (Complex I) in coupled bovine heart submitochondrial particles and in vesicles derived fromParacoccus denitrificans. The inhibitor affects neither NADH oxidase (coupled or uncoupled) nor NADH:ferricyanide reductase and succinate oxidase activities at the concentrations that selectively prevent the succinate‐supported, rotenone‐sensitive NAD+or ferricyanide reduction. Possible uncoupling effects of the inhibitor are ruled out: in contrast to oligomycin and gramicidin, which increases and decreases the rate of the reverse electron transfer, respectively, in parallel with their coupling and uncoupling effects, lauryl sulfate does not affect the respiratory control ratio. A mechanistic model for the unidirectional effect of lauryl sulfate on the Complex I catalyzed oxidoreduction is proposed.