Immunophenotyping of inflammatory cells in lesional skin of the extrinsic and intrinsic types of atopic dermatitis
Immunophenotyping of inflammatory cells in lesional skin of the extrinsic and intrinsic types of atopic dermatitis
复制标题
DOI：
10.1111/j.1365-2133.2004.06027.x
复制
发表时间：
2004-07-01
影响因子：
10.3
通讯作者：
Yang, JM
中科院分区：
文献类型：
--
作者：
Rho, NK;Kim, WS;Yang, JM
背景特应性皮炎(AD)患者中有一部分总IgE水平和特异性IgE水平正常，常见变应原皮肤试验阴性。这种疾病被称为阿尔茨海默病的“固有”形式。目的比较两种类型AD的炎症微环境。方法对18例外源性AD和17例内源性AD患者的皮损和非皮损中的炎性细胞进行免疫表型分析。结果免疫组织化学分析显示两组患者真皮中均有较高比例的CD4+T细胞，且两者的CD4/CD8比值相近。其他T细胞标志物和表皮朗格汉斯细胞的表达水平在两种类型的AD中均升高。尽管两种亚型的T细胞谱系相似，但外源性T细胞和嗜酸性粒细胞颗粒蛋白在真皮中的渗透比内源性T细胞更为明显。结论虽然两种亚型的总体炎症微环境相似，但T细胞细胞因子产生的差异可能是导致这两种亚型组织嗜酸性粒细胞增多的原因之一。
Background There is a subgroup of atopic dermatitis (AD) patients with normal total and specific IgE levels and negative skin tests towards common allergens. This form of the disease has been referred to as the 'intrinsic' form of AD. Although previous studies have demonstrated differences in the cytokine profile between the extrinsic and intrinsic subtypes, the pathogenesis of both subtypes of AD remains unclear.Objectives To compare the inflammatory micromilieu in both forms of AD.Methods Immunophenotyping of the inflammatory cells was performed in lesional and nonlesional skin from 18 patients with extrinsic and 17 with intrinsic AD.Results Immunohistochemical analysis revealed a high proportion of CD4+ T cells in the dermis, with a similar CD4/CD8 ratio in the two groups. The expression levels of other T-cell markers and epidermal Langerhans cells were increased in both forms of AD. Although the T-cell repertoires in the two subtypes were similar, dermal infiltration of eosinophils and eosinophil granular proteins was more prominent in the extrinsic type than in the intrinsic type. Eotaxin immunoreactivity was also significantly higher in the extrinsic subtype.Conclusions The data suggest that although the overall inflammatory microenvironment in the two subtypes appears to be similar, differences in T-cell cytokine production might contribute to the differential tissue eosinophilia in these subtypes.