Alteration of expression pattern of transient receptor potential vanilloid 2 and transient receptor potential vanilloid 3 in ocular surface neoplasm
Alteration of expression pattern of transient receptor potential vanilloid 2 and transient receptor potential vanilloid 3 in ocular surface neoplasm
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DOI：
10.4103/tjo.tjo_12_20
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发表时间：
2020-04
影响因子：
1.1
通讯作者：
Ai Izutani-Kitano;Y. Okada;Kana Ichikawa;P. Reinach;S. Saika
中科院分区：
文献类型：
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作者：
Ai Izutani-Kitano;Y. Okada;Kana Ichikawa;P. Reinach;S. Saika
目的：探讨瞬时受体电位香草素(TRPV)家族成员和Trp ankyrin 1(TRPA1)在正常结膜上皮和病变结膜上皮中的免疫组织化学表达模式是否存在差异。材料和方法：研究对象包括正常结膜上皮、翼状胬肉上皮、上皮异型增生和原位癌。结果：TRPV1、TRPV4和TRPA1分别表达于上述不同上皮层的胞浆和胞核以及胞核和胞浆。这三种色氨酸异构体的表达模式没有差异。另一方面，TRPV2和TRPV3的表达模式在这些不同的受试者之间有很大的差异。TRPV2在正常结膜和翼状胬肉的基底层上皮细胞中均有表达。尽管TRPV2在大多数发育不良的上皮中都不存在，但它的图案散布在这一区域。TRPV2仅在原位癌的部分基底上皮细胞中表达。在正常结膜和翼状疱疹上皮中，TRPV3在所有细胞层的胞浆中均有微弱的表达，在部分基底细胞的胞核中也有微弱的表达，而在所有的异型增生和翼状疱疹上皮细胞核中均有表达。结论：TRPV2和TRPV3的表达模式分析可能是眼表上皮病变的潜在诊断指标。
PURPOSE: We determined if the immunohistochemical expression pattern of transient receptor potential vanilloid (TRPV) family members and TRP ankyrin 1 (TRPA1) differs among a healthy conjunctival epithelium and diseased epithelia. MATERIALS AND METHODS: Subjects include a normal conjunctival epithelium, pterygium epithelium, epithelial dysplasia or carcinoma in situ. RESULTS: TRPV1, TRPV4 or TRPA1 was detected in both the cytoplasm and nuclei, or in either the nuclei or cytoplasm, of these different epithelial layers, respectively. There was no difference in the expression pattern of these three TRP isoforms. On the other hand, the expression patterns of TRPV2 and TRPV3 differed dramatically among these different subjects. TRPV2 was observed in the basal layer epithelium of a normal conjunctiva and pterygium. Its pattern was scattered in this region, although TRPV2 was absent throughout most of the dysplastic epithelium. TRPV2 was detected only in some of the suprabasal epithelial cells of a carcinoma in situ. TRPV3 was faintly detected in the cytoplasm of all the cell layers and also in the nuclei of some of the basal cells in a normal conjunctiva and in the pterygia epithelium, while in situ it was uniformly expressed in all of the dysplasia and carcinoma nuclei in all epithelial cell layers. CONCLUSION: These results suggest that TRPV2 and TRPV3 expression pattern analysis might be potential diagnostic markers of ocular surface epithelial disorders.