Synaptic plasticity in the CA1 area of the hippocampus of scrapie-infected mice
Synaptic plasticity in the CA1 area of the hippocampus of scrapie-infected mice
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DOI：
10.1006/nbdi.1998.0194
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发表时间：
1998-09-01
影响因子：
6.1
通讯作者：
MacLeod, N
中科院分区：
文献类型：
--
作者：
Johnston, AR;Fraser, JR;MacLeod, N
利用常规体外细胞外场电位记录，我们研究了ME7痒病小鼠海马CA1区的短期和长期突触可塑性。与早期的研究一致，在疾病早期（接种后长达160天），未观察到Schaffer侧侧诱发场兴奋性突触后电位的特性发生变化，之后记录的电位可见减弱。此外，到目前为止，配对脉冲促进或破伤风后增强均未见变化，这是与突触前钙水平短暂升高相关的短期现象。然而，从100 d.p.i.开始，切片维持长期增强（LTP）的能力发生了重大变化。在所有这些实验中，短时增强（STP）都被保留了下来，这表明从异常PrP被检测到的时间开始，或者甚至更早，负责稳定LTP维持阶段的机制就受到了损害。这一结果讨论了STP和LTP之间的关系，以及这种关系如何被细胞朊病毒蛋白（PrPC）转化为瘙痒病，蛋白酶抗性PrP （PrPSc）形式所损害。(C) 1998学术出版社。
Using conventional in vitro extracellular field potential recordings we have investigated both short-and long-term synaptic plasticity in the hippocampal CA1 area of mice infected with ME7 scrapie. In agreement with earlier studies, no changes were seen in the properties of the Schaffer collateral-evoked field excitatory postsynaptic potential during the early stages of the disease (up to 160 days, post inoculation, d.p.i) after which time the recorded potentials were seen to attenuate. Also, up to this time no changes were seen in either paired-pulse facilitation or post-tetanic potentiation, which are short-term phenomena associated with brief elevations in presynaptic calcium levels. However, there was a significant shift from the ability of slices to maintain long-term potentiation (LTP) from 100 d.p.i. onwards. In all of these experiments short-term potentiation (STP) was preserved, suggesting that from the time that abnormal PrP becomes detectable, or perhaps even earlier, the mechanisms responsible for stabilizing the maintenance phase of LTP are impaired. This result is discussed in terms of the relationship between STP and LTP and how this might be compromised by the conversion of cellular prion protein (PrPC) to the scrapie, protease resistant form of PrP (PrPSc). (C) 1998 Academic Press.