A STEM-LOOP IN THE 3' UNTRANSLATED REGION MEDIATES IRON-DEPENDENT REGULATION OF TRANSFERRIN RECEPTOR MESSENGER-RNA STABILITY IN THE CYTOPLASM
A STEM-LOOP IN THE 3' UNTRANSLATED REGION MEDIATES IRON-DEPENDENT REGULATION OF TRANSFERRIN RECEPTOR MESSENGER-RNA STABILITY IN THE CYTOPLASM
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DOI：
10.1016/0092-8674(88)90098-0
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发表时间：
1988-06-03
期刊：
影响因子：
64.5
通讯作者：
KUHN, LC
中科院分区：
文献类型：
--
作者：
MULLNER, EW;KUHN, LC
铁剥夺强烈诱导人转铁蛋白受体（hTR）及其mRNA的表达。通过测量转录延伸率，hTR特异性核RNA水平和mRNA半衰期，我们发现这种调节发生在细胞质中的转录后。对3“非翻译区缺失的hTR cDNA突变体的分析揭示了两个不同结构域的存在，这两个结构域都是小鼠L细胞中调控所必需的。调控表型与计算机算法预测的茎环结构的存在相关。油漆和删除突变体的表达影响茎环证实了这种二级结构的监管要求。hTR cDNA的3“非翻译区足以赋予另一个基因铁依赖性调控。
Expression of the human transferrin receptor (hTR) and its mRNA is strongly induced by iron deprivation. By measuring transcription elongation rates, levels of hTR-specific nuclear RNA, and mRNA half-lives, we found this regulation to occur posttranscriptionally in the cytoplasm. Analysis of hTR cDNA mutants with deletions in the 3'' untranslated region revealed the existence of two distinct domains, both of which are essential for regulation in mouse L cells. The regulation phenotype correlates with the presence of a stem-loop structure predicted by a computer algorithm. Expression of paint and deletion mutants affecting the stem-loop confirmed the requirement for this secondary structure in regulation. The 3'' untranslated region of hTR cDNA was sufficient to confer iron-dependent regulation on another gene.