Isothermal desiccation and vitrification kinetics of trehalose-dextran solutions
Isothermal desiccation and vitrification kinetics of trehalose-dextran solutions
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DOI：
10.1021/la0355186
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发表时间：
2004-06-22
期刊：
影响因子：
3.9
通讯作者：
Toner, M
中科院分区：
文献类型：
--
作者：
Aksan, A;Toner, M
干燥状态保存的承诺是基于这样的假设，即降低分子流动性以阻止化学反应和变质速度是干燥样品长期稳定的主要因素。在本研究中，探讨了利用等温、等压玻璃化作为目前使用的保存技术(主要是冷冻保存和冷冻干燥)的一种经济替代技术的可行性。用重量分析、调制差示扫描量热法和X射线结晶学研究了模型海藻糖和海藻糖-葡聚糖体系的干燥和玻璃化动力学。结果表明，海藻糖的玻璃化可以在不结晶的情况下等温实现，加入高相对分子质量的葡聚糖可以显著地加速海藻糖溶液的玻璃化。此外，对于相同的水分含量，海藻糖-葡聚糖溶液的玻璃化转变温度明显高于二元海藻糖溶液的玻璃化转变温度，这使得海藻糖-葡聚糖溶液的玻璃化状态是可能的，储存也是可行的。
The promise of dried state preservation is based on the hypothesis that lowering molecular mobility to halt chemical reaction and deterioration rates is the primary factor for the long-term stability of the dried specimen. In this research, the feasibility of utilizing isothermal, isobaric vitrification as an economical alternative to the preservation technologies currently in use (mainly, cryopreservation and lyophilization) is explored. Desiccation and vitrification kinetics of model trehalose and trehalose-dextran systems were examined using gravimetric analysis, modulated differential scanning calorimetry, and X-ray crystallography. It was shown that vitrification can be achieved isothermally without crystallization and that vitrification of trehalose solutions can be significantly accelerated by incorporating high-molecular-weight dextrans. Additionally, it was shown that, for the same water content, the glass transition temperature of the trehalose -dextran solution is significantly higher than that of the binary trehalose solution, making the glassy state achievable and storage feasible.