Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: Suggestions for appropriate observables
Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: Suggestions for appropriate observables
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DOI：
10.1016/s0006-3495(00)76772-5
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发表时间：
2000-05-01
影响因子：
3.4
通讯作者：
Wilson, KR
中科院分区：
文献类型：
--
作者：
Brown, FLH;Leitner, DM;Wilson, KR
我们考虑了通过单粒子跟踪(SPT)和近场扫描光学显微镜(NSOM)等光学实验来推断细胞骨架与跨膜蛋白相互作用的性质的可能性。特别是，我们证明，通过检测细胞骨架“畜栏”内蛋白质种群的随时间变化的方差和高阶矩，可以区分静态和动态的扩散障碍。模拟红细胞表面带3扩散的模拟提供了一个具体的证明，这些统计工具在生物系统的研究中可能被证明是有用的。
We consider the possibility of inferring the nature of cytoskeletal interaction with transmembrane proteins via optical experiments such as single-particle tracking (SPT) and near-field scanning optical microscopy (NSOM). In particular, we demonstrate that it may be possible to differentiate between static and dynamic barriers to diffusion by examining the time-dependent variance and higher moments of protein population inside cytoskeletal "corrals." Simulations modeling Band 3 diffusion on the surface of erythrocytes provide a concrete demonstration that these statistical tools might prove useful in the study of biological systems.