Centrioles Regulate Centrosome Size by Controlling the Rate of Cnn Incorporation into the PCM
Centrioles Regulate Centrosome Size by Controlling the Rate of Cnn Incorporation into the PCM
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DOI：
10.1016/j.cub.2010.11.011
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发表时间：
2010-12-21
期刊：
影响因子：
9.2
通讯作者：
Raff, Jordan W.
中科院分区：
文献类型：
--
作者：
Conduit, Paul T.;Brunk, Kathrin;Raff, Jordan W.
背景资料：中心体是动物细胞中主要的微管组织中心，由一对中心粒组成，中心粒周围包裹着无定形的中心粒周围物质（PCM）。中心体的大小在细胞周期中受到严格的调控，最近的研究表明，某些干细胞中的两个中心体通常大小不对称。有令人信服的证据表明，中心粒影响中心体的大小，但如何中心体的大小设置仍然mysteries.Results：我们表明，保守的果蝇PCM蛋白Cnn表现出一种不寻常的动态行为，因为Cnn分子只纳入PCM最接近的中心粒，然后通过PCM的其余部分向外传播。Cnn掺入PCM是由与保守的中心粒蛋白Asl（人类中的Cep 152）和DSpd-2（人类中的Cep 192）的相互作用驱动的。Cnn掺入PCM的速率在细胞周期中受到严格调节，并且该速率影响PCM中Cnn的量，这反过来又是整体中心体大小的重要决定因素。有趣的是，女儿在合胞体胚胎中心粒只有开始将Cnn，因为他们脱离他们的母亲，这产生了一个中心体大小的不对称性，与母亲中心粒总是最初组织更多的Cnn比他们的daughter.Conclusions：中心粒可以控制PCM的量，他们组织的调节率Cnn纳入PCM。这一机制可以解释中心体大小在细胞周期中是如何被调节的，也允许母亲和女儿中心粒彼此独立地设置中心体大小。
Background: Centrosomes are major microtubule organizing centers in animal cells, and they comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Centrosome size is tightly regulated during the cell cycle, and it has recently been shown that the two centrosomes in certain stem cells are often asymmetric in size. There is compelling evidence that centrioles influence centrosome size, but how centrosome size is set remains mysterious.Results: We show that the conserved Drosophila PCM protein Cnn exhibits an unusual dynamic behavior, because Cnn molecules only incorporate into the PCM closest to the centrioles and then spread outward through the rest of the PCM. Cnn incorporation into the PCM is driven by an interaction with the conserved centriolar proteins Asl (Cep152 in humans) and DSpd-2 (Cep192 in humans). The rate of Cnn incorporation into the PCM is tightly regulated during the cell cycle, and this rate influences the amount of Cnn in the PCM, which in turn is an important determinant of overall centrosome size. Intriguingly, daughter centrioles in syncytial embryos only start to incorporate Cnn as they disengage from their mothers; this generates a centrosome size asymmetry, with mother centrioles always initially organizing more Cnn than their daughters.Conclusions: Centrioles can control the amount of PCM they organize by regulating the rate of Cnn incorporation into the PCM. This mechanism can explain how centrosome size is regulated during the cell cycle and also allows mother and daughter centrioles to set centrosome size independently of one another.