Electrospun, Reinforcing Network-Containing, Silk Fibroin-Based Nerve Guidance Conduits for Peripheral Nerve Repair
Electrospun, Reinforcing Network-Containing, Silk Fibroin-Based Nerve Guidance Conduits for Peripheral Nerve Repair
复制标题
用于周围神经修复的静电纺丝、包含强化网络、基于丝素蛋白的神经引导导管
DOI：
10.1166/jbt.2016.1417
复制
发表时间：
2016
影响因子：
0.1
通讯作者：
Yumin Yang
中科院分区：
文献类型：
--
作者：
Yaling Wang;Yan Kong;Yahong Zhao;Qilin Feng;Yue Wu;Xin Tang;Xiaosong Gu;Yumin Yang
神经引导导管（NGC）必须具有良好的生物和机械性能，用于周围神经修复应用。在本研究中，我们开发了一种复合结构的静电纺丝丝素蛋白为基础的NGCs（SF-NGCs），通过三个步骤的程序。通过在简单的静电纺丝SF-NGC中嵌入SF基增强网络形成独特的结构，从而导致额外的抗压强度和缝线保持强度。这些复杂的静电纺丝SF-NGCs植入大鼠坐骨神经缺损10毫米长的桥梁。在神经移植后6个月进行组织学和功能评估。功能和形态参数的检测结果表明，这些复杂的静电纺丝SF-NGC促进周围神经再生，因为静电纺丝纳米多孔SF纤维负责其良好的生物学性能和嵌入的增强网络增强其机械性能。本研究表明，SF-NGCs有望成为一种新型的组织工程化神经移植物，用于周围神经修复。
Nerve guidance conduits (NGCs) must have favorable biological and mechanical properties for peripheral nerve repair applications. In this study, we developed a composite structure of electrospun silk fibroin-based NGCs (SF-NGCs) through three step procedures. The unique structure was formed by embedding SF-based reinforcing network in a simple electrospun SF-NGC, thus leading to additional compressive strength and suture retention strength. These complex electrospun SF-NGCs were implanted to bridge a 10-mm long sciatic nerve defect in rats. Histological and functional assessments were performed at 6 months after nerve grafting. Results of examined functional and morphological parameters showed that these complex electrospun SF-NGCs promoted peripheral nerve regeneration because electrospun nanoporous SF fibers were responsible for their good biological properties and an embedded reinforcing network enhanced their mechanical properties. The study suggested that the prepared SF-NGCs may be new type of tissue-engineered nerve grafts for peripheral nerve repair.